女贞叶、茎、根及根际土壤真菌群落结构及功能群特征

doi:10.11707/j.1001-7488.20220511

Abstract

Abstract:

Objective: This study aimed to explore the species composition, diversity and functional group characteristics of fungal community in three vegetative organs and rhizosphere soil of Ligustrum lucidum, so as to lay a foundation for the privet-fungi interaction study and the development and utilization of the privet endophytic and rhizosphere fungal resources.This research could provide a reference for the study of trees microbiome. Method: The leaf, stem, root and rhizosphere soil of L. lucidum were selected as research objects, and the ITS1 region of fungal rRNA gene was sequenced by Illumina MiSeq instrument. Based on the sequencing result, the diversity, species composition, functional group characteristics and their difference in community structure of fungal community in three vegetative organs and rhizosphere soil were analyzed subsequently. Result: The species richness of fungal community of different samples was in the order of RS>SE>RE>LE, and the community diversity was RS>LE>SE>RE. There were 31 OTUs in the four samples, accounting for 2.08% of the total OTUs. Approximately 39.42%, 35.26% and 76.94% of OTUs in leaves, stems and roots were also found in rhizosphere soil, respectively. The β diversity analysis showed that the fungal community structure of leaf was relatively similar to that of stem, but was different from that of root and rhizosphere soil. At the phylum level, the dominant taxon of fungi in the four compartments were Ascomycota and Basidiomycota. At the genus level, the dominant fungi of endophytic fungi community in leaves and stems were Cladosporium and Vishniacozyma while the dominant fungi in the root were Rhexodenticula and Pochonia. In addition, the dominant fungi of rhizosphere soil were Psilocybe and Saitozyma.The common fungi in leaves, stems, roots and rhizosphere soil mainly were Cladosporium, Vishniacozyma, Saitozyma, Rachicladosporium, Symmetrospora, Alternaria and Penicillium. The statistical result of fungi functional group classification showed that the differentiation characteristics of samples could be divided into aboveground and underground parts with the fungi in leaves and stems were mainly of compound trophic (pathotrophy-saprotrophy-symbiotrophy, pathotrophy-saprotrophy), which accounted for 76.97% and 91.68%, respectively, while those were mainly of single trophic fungi (saprotrophy, symbiotrophy) in root and rhizosphere soil, accounting for 60.05% and 67.51%, respectively. Conclusions: The diversity of fungal community in rhizosphere soil is significantly higher than that in leaves, stems and roots of L. lucidum. The fungal community structure and functional group characteristic of leaves are relatively similar to that of stems, but significantly different from those of roots and rhizosphere soil. The fungi functional group in leaves and stems is mostly pathotrophy-saprotrophy-symbiotrophy, while that in root and rhizosphere soil is mostly saprotrophy.

Key words: Ligustrum lucidum, high throughput sequencing, endophytic fungi, rhizosphere fungi, community structure, functional group

CLC Number:

S727.9

Bin Li,Hongxiang Shi,Lanlan Liu,Pu Yang,Xin Zhang,Hang Chen,Ying Feng,Xiaoming Chen. Characteristics of Community Structure and Functional Group of Fungi in Leaf, Stem, Root and Rhizosphere Soil of Ligustrum lucidum[J]. Scientia Silvae Sinicae, 2022, 58(5): 102-112.

Figures/Tables 8

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样品 Sample	原始序列数 Raw sequences	有效序列数 Valid sequences	丰富度指数Richness index		多样性指数Diversity index		覆盖度 Coverage (%)
样品 Sample	原始序列数 Raw sequences	有效序列数 Valid sequences	Chao1	Ace	Shannon	Simpson	覆盖度 Coverage (%)
LE	60 659±5 372	60 527±5381	142.36±28.11 a	138.19±24.56 a	3.08±0.22 a	0.096±0.02 a	99.98±0.01
SE	61 233±4 283	60 331±3 849	283.55±57.84 b	281.89±55.43 b	3.00±0.48 a	0.118±0.02 a	99.93 ±0.04
RE	61 562±9 633	61 330±9 499	191.91±50.66 a	193.38±53.24 a	2.86±0.07 a	0.148±0.06 a	99.97± 0.02
RS	69 180±3 097	67 764±2 814	900.90±12.30 c	903.80±16.31 c	3.89±0.13 b	0.096±0.01 a	99.74 ±0.01
合计Total	757 906	749 855	—	—	—	—	—

样品Sample	R	P
LE vs SE vs RE vs RS	0.898 1	0.001^*
LE vs SE vs RE	0.728 4	0.001^*
LE vs SE vs RS	0.950 6	0.001^*
SE vs RE vs RS	1	0.001^*
LE vs SE	0.777 8	0.098
LE vs RE	1	0.098
LE vs RS	1	0.098
SE vs RE	1	0.098
SE vs RS	1	0.098
RE vs RS	0.814 8	0.098

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Characteristics of Community Structure and Functional Group of Fungi in Leaf, Stem, Root and Rhizosphere Soil of Ligustrum lucidum

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