Scientia Silvae Sinicae ›› 2021, Vol. 57 ›› Issue (3): 29-38.doi: 10.11707/j.1001-7488.20210304
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Pu Zhang1,Wenjing Shao2,Kejiu Du1,3,Shuang Zhang1,3,*
Received:
2020-04-10
Online:
2021-03-25
Published:
2021-04-07
Contact:
Shuang Zhang
CLC Number:
Pu Zhang,Wenjing Shao,Kejiu Du,Shuang Zhang. Cloning and Functional Analysis of Cysteine Proteinase Inhibitor Gene EuCPI from Eucommia ulmoides[J]. Scientia Silvae Sinicae, 2021, 57(3): 29-38.
Table 1
Primers for EuCPI gene cloning"
引物名称 Primer name | 引物序列 Primer sequence(5′—3′) | 引物功能 Primer function |
BS-F | CTCGCTCGTTTCGCTGTC | 中间片段扩增 Intermediate fragment amplification |
BS-R | CCAGACCTTAGCTTCATA | |
3R-OUT | TGAGTTTGTAAGGGTGGTGAAGGC | 3′-RACE |
3R-IN | ACTGTTGAGGCTATTGATGCTGGAAAG | |
5R-OUT | CCAGCATCAATAGCCTCAACAGTAAG | 5′-RACE |
5R-IN | GCCTTCACCACCCTTACAAACTCAAGCAATCC | |
UMP-L | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | 通用引物 Universal primer |
UMP-S | CTAATACGACTCACTATAGGGC | |
ORF-F | CCGGAATTCATGGCAACTCTAGGTGGAC | ORF扩增 ORF amplification |
ORF-R | CCCAAGCTTTTACTCCACTGCACCACCT |
Fig.4
Purification of EuCPI protein and results of Western Blot A: Purification of EuCPI protein M: PageRuler prestained protein marker; 1: pET28a/BL21 bacterial liquid; 2: pET28a-EuCPI/BL21 bacterial liquid; 3-5: 10, 20, 50 mmol ·L-1 imidazole eluate. B: Results of Western Blot M: Non-prestained protein marker; 1: pET28a-EuCPI/BL21 protein; 2: Positive control."
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