Scientia Silvae Sinicae ›› 2020, Vol. 56 ›› Issue (9): 153-163.doi: 10.11707/j.1001-7488.20200917
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Xiaohong Zhao,Shanshan Chai,Manman Zhang,Yichang Fan,Yunfei Mao,Zhiquan Mao,Xiang Shen*
Received:
2019-04-08
Online:
2020-09-25
Published:
2020-10-15
Contact:
Xiang Shen
CLC Number:
Xiaohong Zhao,Shanshan Chai,Manman Zhang,Yichang Fan,Yunfei Mao,Zhiquan Mao,Xiang Shen. Effects of Shell Powder on Microbial Diversity in Acidified Soil and Growth of Malus hupehensis var. mengshanensis Seedlings[J]. Scientia Silvae Sinicae, 2020, 56(9): 153-163.
Table 1
Shell powder with different particle sizes used as soil conditioners"
土壤调理剂 Shell powder conditioners | 成分1 Ingredient 1 | 成分2 Ingredient 2 | 各成分体积比 Ingredients volume proportion | 命名 Name |
贝壳粉处理1 Large grain shell | 大粒贝壳粉 Large grain shell powder | 有机肥 Organic fertilizer | 1:1 | DLB |
贝壳粉处理2 Medium grain shell | 中粒贝壳粉 Medium grain shell powder | 有机肥 Organic fertilizer | 1:1 | ZLB |
贝壳粉处理3 Small grain shell | 小粒贝壳粉 Small grain shell powder | 有机肥 Organic fertilizer | 1:1 | XLB |
对照 Control | 河沙 Sand | 有机肥 Organic fertilizer | 1:1 | CK |
Table 2
Sequencing results of bacteria and fungi in soil samples"
贝壳粉调理剂 Shell powder conditioners | 细菌Bacteria | 真菌Fungi | |||||
原始序列数量 Total tag | 有效序列数量 Effective tag | 有效序列比例 Effective (%) | 原始序列数量 Total tag | 有效序列数量 Effective tag | 有效序列比例 Effective (%) | ||
DLB | 64 913 | 57 604 | 72.04 | 68 323 | 66 897 | 83.65 | |
68 665 | 61 295 | 76.51 | 68 167 | 66 600 | 83.51 | ||
68 306 | 61 356 | 76.61 | 68 933 | 67 481 | 84.02 | ||
ZLB | 68 932 | 61 935 | 77.26 | 68 675 | 67 058 | 84.09 | |
68 594 | 61 647 | 77.07 | 67 106 | 65 497 | 81.89 | ||
68 065 | 61 029 | 76.19 | 68 260 | 66 732 | 83.59 | ||
XLB | 68 153 | 60 824 | 75.90 | 68 450 | 66 881 | 83.53 | |
69 024 | 61 101 | 76.17 | 69 053 | 67 548 | 84.01 | ||
68 516 | 61 697 | 77.18 | 69 028 | 67 454 | 84.43 | ||
CK | 68 597 | 62 178 | 77.78 | 51 749 | 50 616 | 82.69 | |
69 280 | 63 310 | 79.35 | 66 648 | 65 125 | 81.49 | ||
68 766 | 62 539 | 78.38 | 66 654 | 65 102 | 81.45 | ||
总和Total | 819 811 | 736 515 | 76.70 | 801 046 | 782 991 | 83.20 |
Table 3
Soil sample community abundance and diversity index with different shell powder conditioners of different particl sizes"
贝壳粉调理剂 Shell powder conditioners | 细菌Bacteria | 真菌Fungi | |||||||
Ace | Chao | Simpson | Shannon | Ace | Chao | Simpson | Shannon | ||
DLB | 1 837.98±16a | 1 865.08±12a | 0.0047±0.0b | 6.39 ± 0.09a | 380.20±13a | 386.52±14a | 0.25±0.04a | 2.53±0.19 b | |
ZLB | 1 873.62±15a | 1 897.72±18a | 0.0038±0.0b | 6.51±0.08a | 413.32±21a | 419.61±21a | 0.25±0.09a | 2.81±0.52 b | |
XLB | 1 833.93±9a | 1 866.52 ±13a | 0.0044±0.0b | 6.40 ±0.06 a | 419.63±7a | 422.03±6a | 0.15±0.07ab | 3.23±0.38 b | |
CK | 1 217.92±175b | 1 266.29±185b | 0.0084±0.0a | 5.70±0.14 b | 414.82±16a | 414.07±14a | 0.02±0.00b | 4.52±0.06 a |
Fig.7
A histogram of relative abundance at the familyclassification level of bacteria (left), fungi (right) in soil samples One color represents a species, and the color block length represents the relative abundance ratio of the species; for the best view, only the top 10 species of abundance level are displayed, the other species were merged into others to show that the unclassified species represented the species which had not been annotated in taxonomy."
Table 4
Percentage of relative abundance of bacteria and fungi (families) in soil samples %"
分类水平 Classification level | 细菌Bacteria | 分类水平 Classification level | 真菌Fungi | |||||||
CK | DLB | XLB | ZLB | CK | DLB | XLB | ZLB | |||
鞘脂单胞菌科 Sphingomonadaceae | 5.75 | 6.41 | 6.02 | 5.82 | 毛壳菌科 Chaetomiaceae | 3.69 | 47.61 | 41.37 | 49.35 | |
ABS-19科 ABS-19 family | 0.32 | 5.17 | 5.27 | 5.50 | 小囊菌科 Microascaceae | 1.64 | 12.40 | 5.03 | 9.31 | |
酸杆菌科 Acidobacteriaceae | 13.11 | 0.16 | 0.15 | 0.20 | 粪盘菌科 Ascobolaceae | 0.01 | 3.15 | 6.77 | 7.93 | |
亚硝化单胞菌科 Nitrosomonadaceae | 3.22 | 3.24 | 3.79 | 3.17 | 毛球壳科 Lasiosphaeriaceae | 0.85 | 5.81 | 6.35 | 4.32 | |
黄色单胞菌科 Xanthomonadales_ Sedis_Incertae | 0.94 | 4.02 | 4.09 | 3.65 | 丛赤壳科 Nectriaceae | 5.60 | 1.85 | 2.96 | 1.74 | |
厌氧绳菌科 Anaerolineaceae | 0.32 | 3.58 | 4.85 | 3.56 | 肉座菌科 Cordycipitaceae | 0.18 | 6.05 | 3.73 | 0.37 | |
Solibacteraceae | 6.30 | 1.86 | 1.95 | 1.80 | Piskurozymaceae | 2.75 | 3.50 | 1.82 | 1.37 | |
芽单胞菌科 Gemmatimonadaceae | 0.65 | 3.96 | 2.77 | 3.37 | 被孢霉科 Mortierellaceae | 4.39 | 1.97 | 0.82 | 1.02 | |
噬纤维菌科 Cytophagaceae | 0.32 | 2.88 | 3.41 | 3.21 | 假球壳科 Pleosporaceae | 4.33 | 0.07 | 0.26 | 0.12 | |
泉发菌科 Chitinophagaceae | 2.33 | 1.81 | 1.86 | 1.70 | 枝孢霉科 Cladosporiaceae | 3.64 | 0.10 | 0.52 | 0.11 |
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