Scientia Silvae Sinicae ›› 2019, Vol. 55 ›› Issue (10): 19-26.doi: 10.11707/j.1001-7488.20191003
Previous Articles Next Articles
Xinlei Li1,Jiatong Wang1,2,Zhenyuan Sun2,Hengfu Yin1,Zhengqi Fan1,Jiyuan Li1
Received:
2018-12-02
Online:
2019-10-25
Published:
2019-11-22
Supported by:
CLC Number:
Xinlei Li,Jiatong Wang,Zhenyuan Sun,Hengfu Yin,Zhengqi Fan,Jiyuan Li. Anthocyanin Components and Their Relationship with Flower Colors in Petals of Camellia japonica 'Chidan' and Its Bud Mutation Cultivars[J]. Scientia Silvae Sinicae, 2019, 55(10): 19-26.
Table 1
The data of flower colors in Camellia japonica 'Chidan' and its bud mutation cultivars"
CIE L*a*b* 表色系统CIE L*a*b*coordinate | ‘玉丹’ ‘Yudan’ | ‘粉丹’ ‘Fendan’ | ‘赤丹’ ‘Chidan’ | ‘金碧辉煌’ ‘Jinbi huihuang’ | ‘二乔赤丹’1 ‘Erqiao chidan’ 1 | ‘二乔赤丹’2 ‘Erqiao chidan’ 2 |
L* | 93.00 | 67.37 | 56.58 | 32.81 | 71.06 | 55.61 |
a* | -0.82 | 38.65 | 54.31 | 33.00 | 35.65 | 52.47 |
b* | 1.87 | 2.19 | 9.36 | 12.72 | 1.36 | 7.95 |
C* | 2.05 | 38.74 | 55.22 | 35.39 | 35.69 | 53.09 |
h/(°) | 113.44 | 3.11 | 9.52 | 20.93 | 2.07 | 8.61 |
Table 2
Chromatographic and spectral data of anthocyanin components in C. japonica 'Chidan' and its bud mutation cultivars"
色谱峰 Peak No. | 保留时间 Retention time/min | 吸收波长 λmax/nm | A440/Avis-max (%) | 分子离子 Molecular ions (m/z) | 碎片离子 Fragment ions (m/z) | 推定结果 Tentative identification |
P1 | 9.68 | 282、518 | 31 | 449 | 287 | Cy3Ga |
P2 | 10.96 | 283、516 | 32 | 449 | 287 | Cy3G |
P3 | 24.08 | 281、316、517 | 34 | 611 | 449、287 | Cy3GaECaf |
P4 | 27.87 | 283、317、515 | 34 | 611 | 449、287 | Cy3GECaf |
P5 | 28.83 | 284、311、515 | 32 | 595 | 449、287 | Gy3GZpC |
P6 | 29.21 | 282、312、516 | 32 | 595 | 449、287 | Cy3GaEpC |
P7 | 33.32 | 284、314、514 | 32 | 595 | 449、287 | Cy3GEpC |
Table 3
Contents of anthocyanin components in C. japonica 'Chidan' and its bud mutation cultivars mg·g-1 DW"
花青苷成分 Anthocyanin components | ‘玉丹’ ‘Yudan’ | ‘粉丹’ ‘Fendan’ | ‘赤丹’ ‘Chidan’ | ‘金碧辉煌’ ‘Jinbi huihuang’ | ‘二乔赤丹’1 ‘Erqiao chidan’1 | ‘二乔赤丹’2 ‘Erqiao chidan’ 2 |
Cy3Ga | — | 0.351 9±2.18 | 1.257 5±5.88 | 12.878 7±27.18 | 0.200 7±0.83 | 1.123 4±3.35 |
Cy3G | — | 2.266 4±6.85 | 9.529 9±21.37 | 52.477 7±54.28 | 1.760 4±6.76 | 11.665 1±22.69 |
Cy3GaECaf | — | 0.029 5±0.16 | 0.176 0±1.45 | 0.216 9±2.63 | 0.115 1±1.35 | 0.260 4±2.54 |
Cy3GECaf | — | 0.022 2±0.14 | 0.105 1±0.58 | — | 0.108 5±0.65 | 0.154 5±1.24 |
Gy3GZpC | — | 0.257 0±2.11 | 0.427 1±3.26 | 0.283 7±2.38 | 0.381 3±3.19 | 0.667 9±3.45 |
Cy3GaEpC | — | 0.349 6±2.33 | 0.744 9±3.55 | 1.980 6±8.25 | 0.279 6±1.86 | 1.128 3±3.59 |
Cy3GEpC | — | 1.751 8±6.58 | 3.161 7±8.89 | 4.325 1±8.24 | 1.858 4±6.25 | 6.030 8±12.53 |
合计Total | — | 5.028 3 | 15.402 1 | 72.162 6 | 4.704 0 | 21.030 3 |
Table 4
Proportions of anthocyanin components in C. japonica 'Chidan' and its bud mutation cultivars%"
花青苷成分 Anthocyanin components | ‘玉丹’ ‘Yudan’ | ‘粉丹’ ‘Fendan’ | ‘赤丹’ ‘Chidan’ | ‘金碧辉煌’ ‘Jinbi huihuang’ | ‘二乔赤丹’1 ‘Erqiao chidan’ 1 | ‘二乔赤丹’2 ‘Erqiao chidan’ 2 |
Cy3Ga | — | 7.00 | 8.16 | 17.85 | 4.27 | 5.34 |
Cy3G | — | 45.07 | 61.87 | 72.72 | 37.42 | 55.47 |
Cy3GaECaf | — | 0.59 | 1.14 | 0.30 | 2.45 | 1.24 |
Cy3GECaf | — | 0.44 | 0.68 | — | 2.31 | 0.73 |
Gy3GZpC | — | 5.11 | 2.77 | 0.39 | 8.11 | 3.18 |
Cy3GaEpC | — | 6.95 | 4.84 | 2.74 | 5.94 | 5.36 |
Cy3GEpC | — | 34.84 | 20.53 | 5.99 | 39.51 | 28.68 |
高继银, 陈邵云, 徐碧玉. 世界名贵茶花. 杭州: 浙江科学技术出版社. 1999. | |
Gao J Y , Chen S Y , Xu B Y . The world's best camellia cultivars. Hangzhou: Zhejiang Science and Technology Publishing House. 1999. | |
高继银, 苏玉华, 胡羡聪. 国内外茶花名种识别与欣赏. 杭州: 浙江科学技术出版社. 2007. | |
Gao J Y , Su Y H , Hu X C . The identification and appreciation of the world's outstanding camellias. Hangzhou: Zhejiang Science and Technology Publishing House. 2007. | |
管开云, 李纪元, 王仲朗. 中国茶花图鉴. 杭州: 浙江科学技术出版社. 2014. 395- 406. | |
Guan K Y , Li J Y , Wang Z L . Camellias of China. Hangzhou: Zhejiang Science and Technology Publishing House. 2014. 395- 406. | |
张洁, 李崇晖, 王亮生, 等. 植物花青苷液质联用方法的分析鉴定. 食品安全质量检测学报, 2013. 4 (3): 760- 768. | |
Zhang J , Li C H , Wang L S , et al. Golden rules of separation and characterization of plant anthocyanins by high pressure liquid chromatography-tandem mass spectrometry. Journal of Food Safety and Quality, 2013. 4 (3): 760- 768. | |
张洁, 王亮生, 高锦明, 等. 贴梗海棠花青苷组成及其与花色的关系. 园艺学报, 2011. 38 (3): 527- 534. | |
Zhang J , Wang L S , Gao J M , et al. Identification of anthocyanins involving in petal coloration in Chaenomeles speciosa cultivars. Acta Horticulturae Sinica, 2011. 38 (3): 527- 534. | |
钟培星, 王亮生, 李珊珊, 等. 芍药开花过程中花色和色素的变化. 园艺学报, 2012. 39 (11): 2271- 2282. | |
Zhong P X , Wang L S , Li S S , et al. The Changes of floral color and pigments composition during the flowering period in Paeonia lactiflora Pallas. Acta Horticulturae Sinica, 2012. 39 (11): 2271- 2282. | |
Byamukama R , Jordheim M , Kiremire B , et al. Anthocyanins from flowers of Hippeastrum cultivars. Scientia Horticulturae, 2006. 109 (3): 262- 266.
doi: 10.1016/j.scienta.2006.05.007 |
|
Downey M O , Rochfort S . Simultaneous separation by reversed-phase high-performance liquid chromatography and mass spectral identification of anthocyanins and flavonols in Shiraz grape skin. Journal of Chromatography A, 2008. 1201 (1): 43- 47. | |
Fossen T , Andersen M . Cyanidin 3-O-(6'-succinyl-glucopyranoside) and other anthoyanins from Phragmites australis. Phytochemistry, 1998. 49 (1): 1065- 1068. | |
Harborne J B . Spectral methods of characterizing anthocyanins. Biochemical Journal, 1958. 70 (1): 22- 28. | |
Hashimoto F , Tanaka M , Maeda H , et al. Changes in flower coloration and sepal anthocyanins of cyanic Delphinium cultivars during flowering. Bioscience Biotechnology and Biochemistry, 2002. 66 (8): 1652- 1659.
doi: 10.1271/bbb.66.1652 |
|
Hayashi K , Abe Y . Studien über anthocyane, XXIII. Papier-chromatographische übersicht der anthocyane im pfanzenreich Ⅰ. Miscellaneous Reports of the Research Institute for Natural Resources, 1953. 29, 1- 8. | |
Li J B , Hashimoto F , Shimizu K , et al. Anthocyanins from red flowers of Camellia reticulata L. Bioscience Biotechnology and Biochemistry, 2007. 71 (11): 2833- 2836.
doi: 10.1271/bbb.70124 |
|
Li J B , Hashimoto F , Shimizu K , et al. Anthocyanins from red flowers of Camellia cultivar 'Dalicha'. Phytochemistry, 2008a. 69 (18): 3166- 3171.
doi: 10.1016/j.phytochem.2008.03.014 |
|
Li J B , Hashimoto F , Shimizu K , et al. Anthocyanins from the red flowers of Camellia saluenensis Stapf ex Bean. Journal of the Japanese Society for Horticultural Science, 2008b. 77 (1): 75- 79.
doi: 10.2503/jjshs1.77.75 |
|
Li J B , Hashimoto F , Shimizu K , et al. A new acylated anthocyan inform the red flowers of Camellia hongkongensis and characterization of anthocyanins in the Section Camellia species. Journal of Plant Ecology, 2009. 51 (6): 545- 552. | |
Lopes-da-Silva F , Escribano-Bailón M T , Pérez-Alonso J J , et al. Anthocyanin pigments in strawberry. LWT-Food Science and Technology, 2007. 40 (2): 374- 382.
doi: 10.1016/j.lwt.2005.09.018 |
|
Mikanagi Y , Saito N , Yokoi M , et al. Anthocyanins in flowers of genus Rosa, sections Cinnamomeae(=Rosa ), Chinenses, Gallicanae and some modern garden roses. Biochemical Systematics and Ecology, 2000. 28 (9): 887- 902.
doi: 10.1016/S0305-1978(99)00127-1 |
|
Oh Y S , Lee J H , Yoon S H , et al. Characterization and quantification of anthocyanins in grape juices obtained from the grapes cultivated in Korea. Journal of Food Science, 2008. 73 (5): 378- 389.
doi: 10.1111/j.1750-3841.2008.00756.x |
|
Saito N , Yokoi M , Yamaji M , et al. Cyanidin-3-p-coumaroylglucoside in Camellia species and cultivars. Phytochemistry, 1987. 26 (10): 2761- 2762.
doi: 10.1016/S0031-9422(00)83587-6 |
|
Sakata Y , Arisumi K . Cyanidin 3-galactoside, a new anthocyanin from Camellia japonica ssp.rusticana (Honda) Kitamura and its occurrence in the garden forms of Camellia of Japanese origin . Journal of the Japanese Society for Horticultural Science, 1986. 55 (1): 82- 88.
doi: 10.2503/jjshs.55.82 |
|
Sakata Y , Arisumi K . Constitution of anthocyanins in flowers of the wild forms of section Camelllia of Japanese and formosan origin. Journal of the Japanese Society for Horticultural Science, 1987. 56 (2): 208- 214.
doi: 10.2503/jjshs.56.208 |
|
Sakata Y , Arisumi K . Anthocyanins in Camellia polyodonta, C. semiserrata and C. chekiangoleosa, and their phylogenic positions in section Camellia. Journal of the Japanese Society for Horticultural Science, 1992. 61 (2): 375- 381.
doi: 10.2503/jjshs.61.375 |
|
Terahara N , Takeda Y , Nesumi A , et al. Anthocyanins from red flower tea (Benibana-cha), Camellia sinensis. Phytochemistry, 2001. 56 (2): 359- 361. | |
Tomás-Barberán F A , Harborne J B , Self R . Dimalonated anthocyanins from the flowers of Salvia splendens and S. coccinea. Phytochemistry, 1987. 26 (10): 2759- 2760.
doi: 10.1016/S0031-9422(00)83586-4 |
|
Uddin A F M , Hashimoto F , Miwa T , et al. Seasonal variation in pigmentation and anthocyanidin phenetics in commercial Eustoma flowers. Scientia Horticulturae, 2004. 100 (1): 103- 115. | |
Wang L S , Heshimoto F , Shiraishi A , et al. Chemical taxonomy in Xibei tree peony from China by floral pigmentation. Journal of Plant Research, 2004. 117 (1): 47- 55.
doi: 10.1007/s10265-003-0130-6 |
|
Wang L S , Shiraishi A , Hashimoto F , et al. Analysis of petal anthocyanins to investigate flower coloration of Zhongyuan (Chinese)and Daikon Island (Japanese) tree peony cultivars. Journal of Plant Research, 2001. 114 (1113): 33- 43. | |
Wu X L , Prior R L . Systematic identification and characterization of anthocyanins by HPLC-ESI-MS/MS in common foods in the United States:fruits and berries. Journal of Agricultural and Food Chemistry, 2005. 53 (7): 2589- 2599.
doi: 10.1021/jf048068b |
|
Zhang J , Wang L S , Gao J M , et al. Rapid separation and identification of anthocyanins from flowers of Viola yedoensis and V. prionantha by high-performance liquid chromatography-photodiode array detection-electrospray ionisation mass spectrometry. Phytochemical Analysis, 2012. 23 (1): 16- 22. | |
Zhang J , Wang L S , Gao J M . Determination of anthocyanins and exploration of relationship between their composition and petal coloration in crape myrtle (Lagerstroemia hybrid). Journal of Integrative Plant Biology, 2008. 50 (5): 581- 588.
doi: 10.1111/j.1744-7909.2008.00649.x |
[1] | Zhang Qin, Xu Zongda, Zhao Kai, Li Xiaowei, Zhang Luosha, Zhang Qixiang. Isolation and Biological Function Analysis of Anthocyanin Regulatory Gene PmMYB1 from Prunus mume [J]. Scientia Silvae Sinicae, 2018, 54(10): 64-72. |
[2] | Cao Yabing, Zhai Xiaoqiao, Deng Minjie, Zhao Zhenli, Fan Guoqiang. Relationship between Metabolites Variation and Paulownia Witches' Broom [J]. Scientia Silvae Sinicae, 2017, 53(6): 85-93. |
[3] | Yu Jian, Zhao Aichun, Liu Changying, Liang Yanmei, Zhu Panpan, Cai Yuxiang, Wang Xiling, Li Zhengang, Yu Maode. Effects of Exogenous Ethylene and 1-MCP Treatments on the Expression of Genes Involved in Ethylene and Anthocyanin in Mulberry Fruit [J]. Scientia Silvae Sinicae, 2017, 53(2): 138-148. |
[4] | Liu Changying, Li Jun, Zhao Aichun, Wang Xiling, Lü Ruihua, Wang Xiaohong, Lu Cheng, Yu Maode. Changes of Anthocyanin and Chlorophyll Content, and Expression Levels of Related Genes during Development Process of Mulberry Fruit [J]. Scientia Silvae Sinicae, 2014, 50(9): 59-66. |
[5] | Shi Qianqian, Zhou Lin, Wang Yan. Isolation and Expression Analysis of GST Gene Encoding Glutathione S-Transferase of Paeonia delavayi var. lutea Wild Population in Yunnan [J]. Scientia Silvae Sinicae, 2014, 50(12): 63-72. |
[6] | Li Xuefei;Han Tiantian;Dong Yan;Wu Man;Shen Xiang. Relationships between Spectral Reflectance and Pigment or Nitrogen Concentrations in Leaves of Padus virginiana‘Schubert’ [J]. Scientia Silvae Sinicae, 2011, 47(8): 75-81. |
[7] | Ge Yuxuan;Wang Liangsheng;Zhou Xiaohong;Gan Changqing. Correlation between the Leaf Color and Pigments Composition of Cotinus coggygria in Fragrant Hills Park and Their Temporal and Spatial Variation [J]. Scientia Silvae Sinicae, 2011, 47(4): 38-42. |
[8] | Xu Lili;Jiang Weibing;Han Jian;Weng Mangling;Cheng Chunyan;Hua Xiangping. Effects of Foliage Spray of KH2PO4 and Socrose Solution on Changes of Pigments and Net Photosynthetic Rate in Leaves of Red-leaf Peach in Early Summer [J]. Scientia Silvae Sinicae, 2011, 47(3): 170-174. |
[9] | Li Xuefei;Hu Jingjing;Wang Qingju;Shen Xiang;Mao Zhiquan. Effects of Spraying Microelement on Anthocyanin and the Relevant Biosynthesis Enzymes in Prunus persica f. atropurpurea Leaves [J]. Scientia Silvae Sinicae, 2010, 46(12): 75-79. |
[10] | Wen Qiaofu;Shen Hongxiang;Yao Yuncong;Tian Ji;Song Tingting. Cloning and Expression of McDFR gene in the Different Foliar Color Cultivars of Maluscrabapple [J]. Scientia Silvae Sinicae, 2010, 46(11): 16-24. |
[11] | Geng Hui;Shen Hongxiang;Yao Yuncong;Tian Ji;Song Tingting. Cloning of McCHI Gene of Malus Crabapple and Its Expression Analysis in the Cultivars with Different Type Foliar Color [J]. Scientia Silvae Sinicae, 2010, 46(10): 42-49. |
[12] | Feng Lijuan;Yuan Zhaohe;Yin Yanlei;Zhao Xueqing;Xu Xinke;Xu Rong;Li Zifeng. Anthocyanin Content and the Relevant Enzymes Activities during Leaf Color Changing of Two Acer Species [J]. Scientia Silvae Sinicae, 2009, 12(8): 56-60. |
[13] | Zhou Xiaohong;Ge Yuxuan;Wang Liangsheng;Gan Changqing. Physiological Changes during Leaf Color Change in Cotinus coggygria and Effects of Growth Regulators [J]. Scientia Silvae Sinicae, 2009, 12(7): 59-62. |
[14] | Wang Qingju;Li Xiaolei;Wang Lei;Sun Fanya;Shen Xiang. Dynamic Changes of Anthocyanin and the Relevant Biosynthesis Enzymes in Padus virginiana 'Schubert' Leaves [J]. Scientia Silvae Sinicae, 2008, 44(3): 45-49. |
[15] | Tang Qianrui;Chen Defu;Chen Youyun;Zhang Hongzhi;Zhou Puhua. Changes of Physiology and Biochemistry during Leafcolor Transformation in Loropetalum chinense var. rubrum [J]. Scientia Silvae Sinicae, 2006, 42(02): 111-115. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||