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Scientia Silvae Sinicae ›› 2012, Vol. 48 ›› Issue (9): 95-98.doi: 10.11707/j.1001-7488.20120915

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Hypovirulence Strains and Its Transmissible dsRNA of Cryphonectria parasitica

Piao Chungen1, Li Yong1, Lee Sanghyun2, Kim Kyunghee2, Lee Seungkyu2, Guo Minwei1   

  1. 1. Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, The Key Open Laboratory of Forest Protection State Forestry Administration-The Laboratory Beijing 100091;2. Department of Forest Diseases and Pests, Korea Forest Research Institute Seoul 130-712
  • Received:2012-05-03 Revised:2012-07-10 Online:2012-09-25 Published:2012-09-25

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Abstract:

Chestnut blight disease caused by Cryphonectria parasitica is widely distributed throughout chestnut tree plantations in the world.Two hypovirulent strains were selected from 60 strains of C. parasitica by abnormal morphology detection such as reduced conidiation, reduced mycelial growth on PDA medium, and colony color on Bavendamm's medium. Double stranded RNA (dsRNA) was detected from the two hypovirulent strains. The vegetable compatibility group test of twenty virulent strains and one hypovirulent strain (KCP-22) was conducted, and the results showed that only hypovirulent strain KCP-22 and virulent strain KCP-9 could be vegetable compatibility, and the amount of transmitted dsRNA and culture characteristics of the vegetable compatibility strains were changed.

Key words: Cryphonectria parasitica, dsRNA, hypovirulence, chestnut blight

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