杉木炭疽病拮抗菌HY32的发酵条件优化

doi:10.11707/j.1001-7488.20141213

Abstract

Abstract:

Bacterium HY32 was identified as an antagonistic strain against Colletotrichum gloeosporioides previously. Both the fermentation medium formula and culture conditions for the antagonistic bacterium HY32 were optimized by using a single factor test and a response surface methodology (RSM) in this study. The test results indicated that the optimized carbon source was glucose, nitrogen source was yeast extract and peptone, and inorganic salt was NaCl. Based on the single facfor test, the optimized concentration of these four major media components was determined. The OD₆₀₀ (optical density at 600 nm) value of cultures after 48 h fermentation was measured to assess the fermentation rate. The analyzed data indicated that the optimal medium was as the following: glucose 19.80 g ·L^-1, yeast extract 12.16 g ·L^-1, peptone 4.22 g ·L^-1 and NaCl 13.13 g ·L^-1. With this optimal medium, we found that the actual OD₆₀₀ value of HY32 culture was able to reach a high level of 1.743 after 48 h fermentation. Single factor test results also showed that the initial pH value, culture temperature and rotation speed were key factors to influence the bacterial growth and inhibitory rate. The best culture conditions were determined as the following: culture temperature 30.52 ℃, pH 8.09 and rotation speed 184.67 r ·min^-1 respectively.

Key words: Cunninghamia lanceolata, antagonistic bacterium HY32, culture conditions, response surface methodology

CLC Number:

S763.15

Li Hongjun, Zhou Guoying, Lu Zongyan, Tan Yimin, Duan Aiguo. Optimization of Fermentation Medium Formula and Culture Conditions for the Antagonistic Bacterium HY32 against Colletotrichum gloeosporioides[J]. Scientia Silvae Sinicae, 2014, 50(12): 94-100.

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Optimization of Fermentation Medium Formula and Culture Conditions for the Antagonistic Bacterium HY32 against Colletotrichum gloeosporioides

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