Abstract:

To efficiently use lipid of resource insects, petroleum ether was used as impregnant to extract lipid from Tenebrio molitor. Aiming at optimization of the extraction of T. molitor lipid, one-factor tests was firstly used respectively to test temperature, duration and solvent/material ratio effect. And then on the basis of previous results and according to the Box Benhnken test design principles, the model of response surface methodology(RSM) with 3 factors and 3 levels was adopted. Response surface and contour were finally graphed with the extraction rate of T. molitor lipid as the response value. The results showed that the optimum conditions of T. molitor lipid extraction were: extraction temperature: 87.9 ℃, extraction time: 10.1 h, extraction ratio of material to solvent: 2∶50. The real extraction rate of T. molitor lipid under these conditions was up to 31.51%, and it was 2.34% higher than the best result of one-factor tests. Technigues of lipid extraction were improved by response surface methodology, the extraction rate was higher. The result suggest that the new method is effective and economical.

Key words: resource insect, Tenebrio molitor, lipid, extraction, response surface methodology