秦岭地区核桃自然群体和栽培群体的遗传多样性及其演化关系——基于nrDNAITS序列分析

doi:10.11707/j.1001-7488.20141207

Abstract

Abstract:

A total of 359 rDNA ITS sequences were used to study the genetic diversity, population genetic structure and the geographical distribution of haplotype patterns of Juglans regia wild and domesticated populations. The results showed that: based on ITS sequences, 18 haplotypes were found in 29 populations. Nueleotide diversity (π) at species level was 0.005 5,and haplotype diversity(H_d)was 0.274. The haplotype diversity was from 0 to 0.679 in different populations. The gene flow (N_m=48.62) was fairly strong between wild and domesticated populations. Haplotype of network showed that haplotype 7 and haplotype 14 were relatively original in this study. Also, haplotype 7 and haplotype 14 were shared among different populations in wild and domesticated populations. The results of Neutrality test and mismatch distribution analysis showed that common walnut trees have not been expanded in recent history. The result of AMOVA depended on ITS showed that genetic variation of J. regia mainly occurred within wild populations, G_ST was 0.067. The maximum likelihood (ML) tree and neighbor-joining (NJ) tree showed all haplotypes and populations were divided into two clusters.

Key words: Juglans regia, Qinling Mountains, population genetic structure, ITS sequence, gene flow

CLC Number:

Hu Yiheng, Dang Meng, Zhang Tian, Luo Guichun, Xia Hailong, Zhou Huijuan, Hu Dongfeng, He Liang, Ma Zhenhua, Zhao Peng. Genetic Diversity and Evolutionary Relationship of Juglans regia Wild and Domesticated Populations in Qinling Mountains Based on nrDNA ITS Sequences[J]. Scientia Silvae Sinicae, 2014, 50(12): 47-55.

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Genetic Diversity and Evolutionary Relationship of Juglans regia Wild and Domesticated Populations in Qinling Mountains Based on nrDNA ITS Sequences

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