旱柳非叶光合组织(皮层)叶绿体光合特性

doi:10.11707/j.1001-7488.20141104

Abstract

Abstract:

In order to investigate the photosynthetic characteristics of cortex of Salix matsudana branches, this study measured the photosynthetic pigment content of current year branches and fully expanded leaves of S. matsudana cultured in artificial climate chamber. The two kinds of chloroplasts were isolated by sucrose density gradient centrifugation, and their intact rate was measured and their differences between light response characteristics and chlorophyll fluorescence characteristics were analyzed comparatively. The result showed that the chlorophyll content and carotenoid content of the cortex were approximately 10.0% and 13.7% of that from the leaf, respectively. The ratio of chlorophyll-a to chlorophyll-b in the cortex was lower than that of the leaf, and the ratio of chlorophyll to carotenoid was conversely. The intact rate of acquired chloroplasts was more than 80%, which was able to meet the needs of research on photosynthetic physiological characteristics of chloroplasts. The rate of photosynthetic oxygen evolution of cortical chloroplasts was lower than that of leaf chloroplasts all the way under the light intensity of 100, 200, 300, 500, 800 μmol·m^-2s^-1, respectively. The light saturation point of cortical chloroplasts was at 300 μmol·m^-2s^-1 with the maximum rate of photosynthetic oxygen evolution of 35.58 μmol·mg^-1 Chl h^-1 while the leaf was at 500 μmol·m^-2s^-1 with the maximum rate of photosynthetic oxygen evolution of 42.78 μmol·mg^-1Chl h^-1, respectively. The maximum photochemical efficiency of PSⅡ(F_v/F_m) of cortical chloroplasts was greater than that of leaf chloroplasts by 30.2% averagely, and the potential photochemical activity of PSⅡ(F_v/F_o) was greater than the leaf chloroplasts by 36.1% averagely. In summary, the photosynthetic rate and light saturation point of cortical chloroplasts of S. matsudana were lower than those of the leaves, however the cortical chloroplast had characteristics such as higher light energy conversion efficiency and lower chlorophyll a/b ratio which was adapted to the light environment within the branches.

Key words: Salix matsudana, cortex, chloroplast, photosynthetic characteristics

CLC Number:

S718.43

Zhang Jinyao, Liu Junxiang, Jü Guansheng, Han Lei, Sun Zhenyuan. Chloroplast Photosynthetic Characteristics of Non-Leaf Photosynthetic Tissues (Cortex) of Salix matsudana[J]. Scientia Silvae Sinicae, 2014, 50(11): 30-35.

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Chloroplast Photosynthetic Characteristics of Non-Leaf Photosynthetic Tissues (Cortex) of Salix matsudana

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