暗孢耳霉侵染竹梢凸唇斑蚜的生物学特征及毒力测定

doi:10.11707/j.1001-7488.20141010

Abstract

Abstract:

Bamboo forests are multi-function resources, which frequently suffer from bamboo aphid infestation. This study aimed to estimate the potential of the aphid-specific pathogen Conidiobolus obscurus on bamboo aphid biocontrol. Tthe fungal infection features on Takecallis taiwanus were observed by scanning electronic microscopy. Conidia adherent to the aphid cuticle could germinate rapidly to invade into host body. 24 h after inoculation, first cadavers appeared. Cystidia penetrated the cuticle in 6 h after host death, and followed by conidiophores, which sporulate for renewing next infection cycle. The result of multi-concentration bioassay showed that C. obscurus had high infectivity to T. taiwanus. After inoculated at high conidial concentration of (192.6±20.3) conidia·mm^-2, 92.8% aphids died in 5 days. The bioassay data were well fitted to the time-concentration-mortality model, and the lowest value of the median lethal concentration was computed as 51.6 conidia·mm^-2 on day 5. Meanwhile, the median lethal time was computed as 2.3 days under the inoculation of 100 conidia·mm^-2. All the results presented the promising potential of C. obscurus on bamboo aphid pest control.

Key words: Conidiobolus obscurus, Takecallis taiwanus, bioassay, scanning electronic microscopy, biocontrol

CLC Number:

S763.3

Wang Dawei, Ma Liangjin, Zhou Xiang. Infection Features and Bioassay of Aphid-Obligate Conidiobolus obscurus on the Bamboo Aphid Takecallis taiwanus[J]. Scientia Silvae Sinicae, 2014, 50(10): 74-79.

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Infection Features and Bioassay of Aphid-Obligate Conidiobolus obscurus on the Bamboo Aphid Takecallis taiwanus

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