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Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (7): 105-112.doi: 10.11707/j.1001-7488.20140715

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Phospholipid Fatty Acid Analysis of Soil Microbes at Different Elevation of Wuyi Mountains

Wu Zeyan1,2, Lin Wenxiong1,2, Chen Zhifang3, Liu Jinfu3, Fang Changxun2, Zhang Zhixing2, Wu Linkun2, Chen Ting2   

  1. 1. College of Life Sciences, Fujian Agriculture and Forestry University Fuzhou 350002;
    2. Institute of Agroecology, Fujian Agriculture and Forestry University Fuzhou 350002;
    3. College of Forestry, Fujian Agriculture and Forestry University Fuzhou 350002
  • Received:2013-04-17 Revised:2013-12-14 Online:2014-07-25 Published:2014-07-04
  • Contact: 林文雄

Abstract:

In this study, experimental sites were respectively set in evergreen broadleaf forest(EBF), coniferous forest(CF), subalpine dwarf forest(SDF)and alpine meadow(AM) along an elevational in the national natural reserve of Wuyi Mountains, and the phospholipid fatty acid analysis(PLFA) was used to examine variations in soil microbial community diversity and its influencing factors. The results showed that: There existed 25 PLFAs that were significantly different in the soil samples from different elevations. The EBF soil exhibited richer PLFAs distribution both in type and amount than the other vegetation types. The microbial activity and functional diversity were decreased with increasing elevation, and in an order of EFB>CF>SDF>AM. The PLFAs biomarkers of three species with the highest contents were 18:1ω9c, 16:1ω7c, 16:00 and cy17:0. The bacterium had the richest distribution, followed by fungus and actinomycetes at different elevations. The Simpson index, Shannon-Wiener index, Brillouin index and McIntosh index of EBF were holistically higher than those of AM. Component analysis(PCA)identified 2 principal component factors in relation to microbial community diversity, explaining 59.23% and 35.46% of the variation, respectively. The PLFAs, which play a major role on first principal component, are 16:1ω9c,a17:0,18:1ω9c and 16:1ω7c, and, which play a major role on second principal component, are i17:0,16:1ω5c and cy17:0. The PLFAs of different microbial groups were closely correlated to the soil physicochemical property and enzyme activities, and the TOC, TN and catalase were positively correlated with the PLFAs of bacteria, actinomycete and protozoon. The soil microbial community diversity was decreased along the elevation in Wuyi Mountain, and vegetative biomass, forest litter, soil nutrient, microzoon and plant roots might be the major factors controlling soil microbial community diversity.

Key words: PLFA, community structure, elevation, soil microorganism, Wuyi Mountains

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