Abstract:

The pollen grains of wild Rosa rugosa from Shandong (MP, RCH), Liaoning (ZHH) and Jilin province (HCW, HCN, HCP) were examined under Scanning Electron Microscope (SEM). The results showed that the pollen grains of wild Rosa rugosa were prolate or perprolate, and their equatarial view was long ellipse, long ellipse leaning toward rectangle or ellipse leaning toward spindle and the polar view was 3-labed-rounded, the two poles were plane or embowed. They all had 3 colposates which annularly distributed in equal space, and the pollens belonged to N₃P₄C₅ model. The sculptural type of the wild Rosa rugosa was striate-foveolate with many stripes and tectum punctures in the exine sculptures. It was worth to note that the morphology and distribution of the stripes and punctures were markedly different among 6 wild materials, and the exine sculpture could be the important evidence for classification and identification of wild Rosa rugosa. The clustering analysis of the pollen characteristics suggested that the relationship between HCW and HCN was close, as well as MP, ZHH and RCH, whereas the relationship between HCP and the other 5 wild materials were very distant. Finally, the origin and evolution of wild Rosa rugosa in china were discussed in terms of the clustering result and the evolution rules of botanic pollen.

Key words: Wild Rosa rugosa, pollen morphology, clustering analysis, origin, evolution