HSP ∷ FT转基因杨树热激开花的影响因素

doi:10.11707/j.1001-7488.20140110

Abstract

Abstract:

In order to understand the underline mechanism for inducing normal flowering efficiently by HSP ∷ FT construct on forest trees, transgenic poplar plants (Populus tremula × P. tremuloides) containing HSP ∷ FT 1 were used as the experimental material to investigate factors affecting FT gene expression to induce precociously flowering of poplar by heat shock treatment in this paper. The results showed that the factors, such as transgenic lines and ramets, plant size and age, heat shock temperature, heat induction duration, and environment temperature, all affected FT gene expression to induce flowering of transgenic plants. Different transgenic lines and different ramets in the same line varied significantly in the induced flowering. Plants with a height lower than 30 cm failed to flower. Flowering frequency increased with increasing height of plants. Heat shock temperature affected initial flowering time, flowering frequency and normal catkin yields. The optimum temperature was 40℃. Flowering of transgenic plants by heat shock was mainly influenced by daily heat induction hours at early stages and by heat induction duration at later stages. The continue heat treatment for more than 3 weeks was efficient to induce flowering. It was beneficial to flowering induction and normal catkin development if transgenic plants grew at lower environment temperature before heat treatment.

Key words: FT gene, poplar, heat shock induction, precociously flowering, influence factors

CLC Number:

Jia Xiaoming, Zhang Huanling, Tang Ming. Factors Influencing the Heat Shock Flowering of HSP ∷ FT Transgenic Poplar[J]. Scientia Silvae Sinicae, 2014, 50(1): 63-68.

References

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Factors Influencing the Heat Shock Flowering of HSP ∷ FT Transgenic Poplar

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