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Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (6): 35-42.doi: 10.11707/j.1001-7488.20100606

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Isolation and Expression of the Chloroplast Copper/Zinc Superoxide Dismutase Gene (GbCuZnSOD ) from Ginkgo biloba

Li Linling1,2;Cheng Hua2;Xu Feng3;Wang Yan3;Jiang Dezhi3;Cheng Shuiyuan2   

  1. 1.College of Horticulture Science,Hebei Agricultural UniversityBaoding 071001;2.College of Life Science and Engineering,Huanggang Normal UniversityHuanggang 438000;3.College of Horticulture and Gardening,Yangtze UniversityJingzhou 434025
  • Received:2009-04-13 Revised:2009-10-20 Online:2010-06-25 Published:2010-06-25

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Abstract:

A full-length cDNA sequence of Ginkgo biloba chloroplast copper/zinc-superoxide dismutase gene(GbCuZnSOD ,FJ555020) was isolated from G.biloba for the first time.The full-length cDNA of GbCuZnSOD was 783 bp,including a 642 bp open reading frame (ORF) which was deduced to encode a 213-amino-acid protein.A predicted molecular weight of the protein was 21.8 ku with pI of 6.82.Three-dimension structure modeling showed that GbCuZnSOD contained 3 helixes and 8 sheets,and had a cylinder motif and three external loops in the enzyme core.Phylogenetic tree analysis revealed that GbCuZnSOD shared the same ancestor with other CuZnSODs.Bioinformatic analysis showed that the CuZnSOD contained a chloroplast-targeted transit peptides.Southern analysis showed that the GbCuZnSOD genes was encoded by a small gene family in G.biloba .Northern hybridization analysis showed that GbCuZnSOD expressed in leaves,stems and fruits,with the highest expression in leaves.There was no expression found in roots.The expression of GbCuZnSOD could be induced by ABA and 36 ℃ temperature,but not by osmoticums and low temperature.

Key words: Ginkgo biloba, copper/zinc-superoxide dismutase gene (CuZnSOD), expression analysis