枣基因组的微卫星特征

doi:10.11707/j.1001-7488.20131212

Abstract

Abstract:

In this study, the genome of Ziziphus jujuba was partially sequenced using the Roche 454 FLX sequencer. The assembled sequences were totally 8.4 Mb, from which 15 036 microsatellites repeats were detected. Among them, the hexanucleotide repeats were the most abundant (6 033), accounting for 40.1% of the total microsatellites, following by the compound (2 707) and mononucleotide(2 575) repeats that accounted for 18.0% and 17.1% of the total microsatellites, respectively. The number of dinucleotide (1 118), trinucleotide (1 218), tetranucleotide (1 050), and pentanucleotide repeats (335), were found to account for 7.5%, 7.0%, 8.1%, 2.2% of the total microsatellites, respectively. While hexanucleotide repeats were the most abundant, dinucleotide repeats were more frequent than the other types of microsatellites in terms of changes in the number of repeat motifs. In mononucleotide and dinucleotide repeats, A/T and AT/TA were the main repeating motifs, while (AAN)_n, (AAAN)_n, (AAAAN)_n and (AAAAAN)_n were the dominant repeat motifs in tri-, tetra-, penta-and hexanucleotide repeats, respectively. All the dominant repeat motifs in different types of microsatellites were rich in A and T. It is noteworthy that the variation of microsatellites was found to be negatively correlated with the lengths of repeat motifs, which suggested that the microsatellites with longer repeat motifs would change faster than those with shorter repeat motifs. This study provided abundant genome sequences and marker resources for different aspects of genetic studies on Z. jujuba.

Key words: Ziziphus jujuba, genome, 454 sequencing, microsatellite

CLC Number:

S718.46

Ma Qiuyue, Dai Xiaogang, Chen Yingnan, Zhang Defang, Liao Zhuoyi, Li Shuxian. Characterization of Microsatellites in the Genome of Ziziphus jujuba[J]. Scientia Silvae Sinicae, 2013, 49(12): 81-87.

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Characterization of Microsatellites in the Genome of Ziziphus jujuba

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