转抗菌肽基因毛白杨的培育及其对溃疡病的抗性

doi:10.11707/j.1001-7488.20110718

Scientia Silvae Sinicae ›› 2011, Vol. 47 ›› Issue (7): 123-127.doi: 10.11707/j.1001-7488.20110718

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Transformation of an Antimicrobial Peptide Gene LJAMP2 into Populus tomentosa and Resistance of the Transgenic Poplar to Canker Disease

Jia Zhichun, Sun Yimin, Qing Zhichao, Xiao Xunyan, Qi Daihua, Luo Keming

Key Laboratory of Eco-Environments of Three Gorges Reservoir Region of Ministry of Education Institute of Resources Botany, School of Life Sciences, Southwest University Chongqing 400715

Received:2010-03-26 Revised:2010-05-18 Online:2011-07-25 Published:2011-07-25

Abstract

Abstract:

An nsLTPs-like antimicrobial protein gene(LJAMP2)from the seeds of motherwort (Leonurus japonicus)was introduced into Chinese white poplar(Populus tomentosa)via Agrobacterium-mediated transformation using nptII gene conferring kanamycin resistance as a selectable marker. Thirty GUS-positive poplar lines were obtained and stable integration of transgenes in the plant genome was identified by PCR analysis. LJAMP2 expression was detected in transgenic plants by reverse transcription-PCR(RT-PCR). Transgenic poplar lines were tested for resistance to the fungal pathogen Cytospora chrysosperma crude extracts from the transformants showed in vitro inhibitory activity against the fungal pathogen. in vivo assays demonstrated that the transgenic poplar plants displayed a significant reduction in their disease symptoms after infection with C. chrysosperma, compared to the control plants. These results suggested that constitutive expression of the LJAMP2 gene from motherwort could improve resistance to canker disease in Chinese white poplar.

Key words: Populus tomentosa, LJAMP2, transformation, canker disease

CLC Number:

S763.1

Jia Zhichun;Sun Yimin;Qing Zhichao;Xiao Xunyan;Qi Daihua;Luo Keming. Transformation of an Antimicrobial Peptide Gene LJAMP2 into Populus tomentosa and Resistance of the Transgenic Poplar to Canker Disease[J]. Scientia Silvae Sinicae, 2011, 47(7): 123-127.

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Transformation of an Antimicrobial Peptide Gene LJAMP2 into Populus tomentosa and Resistance of the Transgenic Poplar to Canker Disease

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