Abstract:

In order to investigate the expression pattern of genes during rooting of <i>Larix</i> cuttings,two differentially expressed cDNA libraries were constructed by using two <i>Larix</i> clones whose rates of rooting are significantly different.Each 500 clones,stochastically selected from positive and negative library,were sequenced.After sequencing and assembling,we obtained 521 UniEST(272 from positive library and 249 from negative library).The UniEST included the genes related to metabolism,signal pathway,transport,resistance,development and cell structure.The result suggested that these genes might have the function during rooting of <i>Larix</i> cuttings.The representational genes were selected and further detected by using semi-quantitative RT-PCR to understand their expression patterns in roots,stems,leaves and at different stages of rooting of <i>Larix</i> cuttings.The result indicated that hormones played pivotal roles in rooting of <i>Larix</i> cuttings.Besides genes related to hormones,small G protein genes,ATP binding cassette transporters genes and WRCs genes were all involved in the rooting procedure.

Key words: <, i>, Larix<, /i>, SSH library, cutting, auxin