Abstract:

Pre column derivation of and high performance liquid chromatography (HPLC) with ultraviolet detection were used to detect the epurated artemisinin in Artemisia annua, and the artemisinin content and biomass yield were compared between the introduced A. annuaand the local wild A. annua. The result showed that the quantity of artemisinin was mainly affected by the genetic background, and secondly influenced by the environment and climate of the cultivated region. The seedlings raised with the integrated platetype paper containers method in greenhouse had longer growth period of A. annua by about 45 days in frigid area. The highest content of artemisinin was 0.555% and the highest biomass yield was (3 526.7±378) kg·hm^-2. High artemisinin content and biomass yield together contributed to artimisinin production. The introduced cultivars had a strong capacity to quickly adapt to the circumstance in Heilongjiang. The artemisinin contents reached to the purchasing level. The mean biomass of individual plant was greater than that in its original region.

Key words: Artemisia annua, artemisinin, HPLC, content, biomass