Abstract:

In the study,the dynamics of lignolytic and cellulolytic enzymes,viz.laccase,lignin peroxidase(LiP),manganese peroxidase(MnP),carboxymethylcellulase(CMCase)and filter paper activity(FPA)secreted by six soil-inhabiting deuteromycetes,were investigated systematically during the decomposition of Pinus massoniana needle litter in solid state fermentation(SSF).The six deuteromycetes exhibited varied profiles of enzymes secretion and caused different loss in TOM and decomposing rate.Pestalotiopsis sp.produced the highest laccase activity and caused the highest loss in TOM;Alternaria sp.produced the highest MnP activity;the highest CMCase and FPA activities were detected in strain Pestalotiopsis sp.The process of decomposition was closely correlated to the changes of enzyme activities.The early-stage decomposition primarily depended on activities of cellulolytic enzymes.While later-stage decomposition depended on the lignolytic enzymes,which acted synergistically with cellulase.According to these results,fungi could be divided into two functional groups:group 1 were cellulose decomposers,including Alternaria sp.,Penicillium sp.,Cephalosporium sp.and Tricherderma sp.,group 2 were decomposers of lignocellulose,including Pestalotia sp.and Aspergillus fumigatus.Meanwhile,we find Pestalotiopsis sp.could produce an appreciable high level of laccase.

Key words: deuteromycetes, litters, decomposing rate, lignolytic enzymes, cellulolytic enzmes