Abstract:

The fungi of genus Botryosphaeria,genus Coniothyrium and genus Dothichiza can cause huge disaster in forestry and man-made poplar forestry in China.Six micro-satellites primers [(GT)n,(CGA)n,(CCA)n,(GAA)n,(CAA)n,(GA)n] and one mini-satellites primer M13 used to identification the molecular relationship of those three genus.All 42 tested isolated isolates are clustered into 4 groups,22 Botryosphaeria isolates and 6 Dothiorella isolates are disperse in this 4 groups,but group I,Ⅱ and IV formed only by the Botryosphaeria and Dothiorella isolates.However,the other 6 Botryosphaeria dothidea isolates and 4 Botryosphaeria berengeriana isolates formed the subgroup of group Ⅲ,6 Coniothyrium isolates and 2 Dothichiza isolates only occurred in the subgroup of group Ⅲ.The results of RAMs-PCR showed that the Botryosphaeria dothidea or Dothiorella isolates which identification by anamorph characters were not a monophyletic group.The putative pathogenic fungus of apple stem rot and ring spot,B.berengeriana and its physiological form could not formed a clear cluster branch separately and which merging with some B.dothidea isolates in a complex group.This result illustrated that Botryosphaeria berengeriana is very similarly with some B.dothidea isolates.The result also illustrated a close relationship between genus Coniothyrium and Dothichiza.

Key words: Botryosphaeria, Coniothyrium, Dothichiza, Botryosphaeria dothidea, RAMs-PCR, tree canker