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Scientia Silvae Sinicae ›› 2006, Vol. 42 ›› Issue (10): 39-42.doi: 10.11707/j.1001-7488.20061007

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Optimization of Medium Proportion of Ginkgo biloba by Uniform Design

Hu Huilu,Cai Xinling   

  1. Forestry and Landscape College, Anhui Agricultural University Hefei 230036
  • Received:2005-08-15 Revised:1900-01-01 Online:2006-10-25 Published:2006-10-25

Abstract:

The tissue culture of Ginkgo biloba in different young position,such as leaf,stem section and bud,were carried out in the paper,by using MS medium added different concentrations of plant growth regulators for instance 2,4-D,NAA and 6-BA. Taking uniform design U*4(64),both the callus induction and the regeneration of explants with different materials and different hormones were analyzed in the experiment. The result showed that the optimal choice was NAA 2.0 mg·L-1 + 6-BA 1.5 mg·L-1 + bud,and there was a lack of significance of difference for 2,4-D.

Key words: Ginkgo biloba, uniform design, medium proportion, optimization