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Scientia Silvae Sinicae ›› 2005, Vol. 41 ›› Issue (1): 42-48.doi: 10.11707/j.1001-7488.20050109

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QTL Analysis of Leaf Morphology and Spring Bud Flush in （Populus tomentosa×P. bolleana)×P. tomentosa

Zhang Deqiang1,Zhang Zhiyi1,Yang Kai2,Li Bailian3   

  1. 1.Institute of Populus tomentosa, Beijing Forestry University Beijing100083; 2.Institute of Crop Germplasm, Chinese Academy of Agriculture Beijing100081; 3.Department of Forestry, North Carolina State University North Carolina State27695-8203
  • Received:2004-04-16 Revised:1900-01-01 Online:2005-01-25 Published:2005-01-25

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Abstract:

One hundred and twenty progeny of Populus were derived from a cross between female clone “TB01" (Populus tomentosa×P. bolleana) and the male clone “LM50" (P. tomentosa). This population was used to detect quantitative trait loci (QTLs) affecting leaf morphology and spring bud flush. A total of 393 AFLP markers were identified and used to construct a parental specific genetic map using pseudo-test-cross mapping strategy. The total genome length corresponding to 3 265.1 cM for the clone “LM50" map and 1 992 cM for the clone “TB01" map with 4～30 markers per linkage group was obtained. Fourteen QTLs controlling leaf morphology were identified on nine linkage groups, and 3 QTLs affecting spring bud flush were detected on three linkage groups with interval mapping software. The phenotypic variance explained by each QTL ranged from 7.6% (on TBLG14) to 15.8% (on TLG9). Co-localization of QTLs controlling correlated traits such as leafblade length, leafblade width and leafblade area were mainly found on linkage groups TLG2 and TLG11 in the genetic map of clone “LM50", and on linkage group TBLG1 in the genetic map of clone “TB01". Based on the genomic regions of QTLs for leaf morphology and spring bud flush, these two traits seem to be controlled by separate genes.

Key words: Populus tomentosa×, P. bolleana)×, P. tomentosa, QTLs mapping, leaf morphology, spring bud flush