Abstract:

Vertical slab polyacrylamide gel electrophoresis was used to examine inheritance of five isoenzymes in megagametophytes of Taxus Yunnanensis.Genetic diversity and genetic differentiation in three natural populations of Taxus Yunnanensis in Northwestern Yunnan were analyzed at ten loci coding for five enzyme systems.The percentage of loci polymorphism was 0.77.The average observed heterozygosity of all populations was 0.302,and the average expected heterozygosity was 0.320.The average number of alleles detected per locus was 2.05.The effective number of alleles per locus was 1.466.Measurement of gene diversity for ten loci showed that interpopulation genetic differentiation was 14.66%,and about 85% of the total gene diversity resided within population.The mean genetic distance was 0.118.

Key words: Taxus yunnanensis, Isoenzyme, Population, Genetic diversity, Genetic differentiation