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林业科学 ›› 2023, Vol. 59 ›› Issue (1): 99-109.doi: 10.11707/j.1001-7488.LYKX20210213

• 研究论文 • 上一篇    下一篇

拟蕈状芽孢杆菌JYZ-SD5与菌根真菌互作对水杉生长和耐盐性的影响

李振倩,吴小芹*,孔维亮   

  1. 南方现代林业协同创新中心 南京林业大学林学院 南京 210037
  • 收稿日期:2021-03-23 出版日期:2023-01-25 发布日期:2023-02-24
  • 通讯作者: 吴小芹
  • 基金资助:
    国家重点研发计划项目(2017YFD0600104);江苏高校优势学科建设工程项目(PAPD)

Effects of Interaction between Bacillus paramycoides JYZ-SD5 and Mycorrhizal Fungi on Growth and Salt Tolerance of Metasequoia glyptostroboides

Zhenqian Li,Xiaoqin Wu*,Weiliang Kong   

  1. Co-Innovation Center for the Sustainable Forestry in Southern China College of Forestry, Nanjing Forestry University Nanjing 210037
  • Received:2021-03-23 Online:2023-01-25 Published:2023-02-24
  • Contact: Xiaoqin Wu

摘要:

目的: 研究盐胁迫下拟蕈状芽孢杆菌(JYZ-SD5)和裂褶菌(Be)互作对水杉生长和耐盐性的影响, 以探究其作用机制, 为利用有益微生物提高木本植物耐盐性以及盐碱地生物修复利用提供参考依据。方法: 盐胁迫下采用盆栽试验探讨拟蕈状芽孢杆菌JYZ-SD5和裂褶菌Be对水杉相关生理代谢指标和耐盐性的影响。结果: 1) 与对照相比, 各接菌处理均提高了水杉的生物量和根系生长参数, 其中双接种JYZ-SD5+Be效果优于单接种, 双接种处理的水杉幼苗株高和地径分别提高65.47%和63.46%, 根长、根表面积、根体积、总根尖数和分枝数分别显著提高1.05、1.51、2.2、1.88和2.31倍(P<0.05)。2)在盐胁迫下, 与未接种对照相比, 各接菌处理均有效缓解了盐胁迫对水杉叶片的损害, 盐害症状减轻; 单接种JYZ-SD5分别显著提高了水杉可溶性糖、可溶性蛋白和脯氨酸12.4%、62.58%和38.99%(P<0.05), 单接种Be以及双接种处理分别显著提高水杉脯氨酸122%和35.64%(P<0.05)。3)盐胁迫下, 与未接种对照相比, 各接菌处理均有效降低水杉H2O2含量、O2·-产生速率、丙二醛含量和电解质渗漏, 其中单接种JYZ-SD5分别显著降低26.58%、9%、15.85%和18.76%(P<0.05), 单接种Be分别降低8.91%、7.77%、8.54%和22.09%, 双接种JYZ-SD5+Be分别显著降低15.58%、26.38%、53.66%和30.39%(P<0.05);同时各接菌处理均显著降低了水杉的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性。4)盐胁迫下, 与未接种对照相比, 各接菌处理提高了水杉茎和叶中的K+含量, 降低了Na+含量, 从而降低了Na+/K+比, 尤以单接种Be效果最好, 分别显著降低水杉茎和叶Na+/K+比23.84%和26.58%(P<0.05)。结论: 根际促生细菌JYZ-SD5与外生菌根真菌Be可促进水杉生长, 并可通过提高水杉植株水分吸收、渗透调节物质积累、维持细胞膜稳定性、降低膜脂质过氧化水平、活性氧积累及抗氧化酶活以及降低Na+/K+比来缓解盐胁迫对水杉的损伤, 提高水杉对盐胁迫的耐受性。

关键词: 拟蕈状芽孢杆菌JYZ-SD5, 裂褶菌Be, 水杉, 盐胁迫, 耐盐性

Abstract:

Objective: This study investigated the effects of the interaction between Bacillus paramycoides JYZ-SD5 and Schizophyllum commune Be on the growth and salt tolerance of Metasequoia glyptostroboides under salt stress, in order to explore the mechanism of action, and to provide a reference for the use of beneficial microorganisms to improve the salt tolerance of woody plants and the bioremediation of saline-alkali land. Method: A pot experiment was conducted to investigate the effects of B.paramycoides JYZ-SD5 and S.commune Be on the physiological and metabolic indexes and salt tolerance of M.glyptostroboides under NaCl stress. Result: 1) Compared with control seedlings, the biomass and root growth parameters of M.glyptostroboides were improved by different inoculation treatments, and the effect of co-inoculation with JYZ-SD5+Be was better than single inoculation with JYZ-SD5 or Be.The height growth, diameter growth, root length, root surface area, root volume, root tips number and branches of M.glyptostroboides of co-inoculation with JYZ-SD5+Be were significantly increased by 65.47%, 63.46%, and 1.05, 1.51, 2.2, 1.88, and 2.31 times, respectively (P < 0.05).2) Under salt stress, compared with uninoculated control, all inoculation treatments effectively alleviated the damage of salt stress on M.glyptostroboides leaves and relieved the symptoms of salt damage.Single inoculation with JYZ-SD5 improved the soluble sugar, soluble protein and proline significantly by 12.4%, 62.58%, and 38.99%, respectively (P < 0.05), single inoculation with Be and co-inoculation with JYZ-SD5+Be significantly improved proline by 122% and 35.64%(P < 0.05).3) Under salt stress, compared with uninoculated control, all inoculation treatments effectively reduced the H2O2 content, O2·-generation rate, MDA content and electrolyte leakage, among which, single inoculation with JYZ-SD5 significantly reduced by 26.58%, 9%, 15.85%, and 18.76%(P < 0.05), single inoculation with Be reduced by 8.91%, 7.77%, 8.54%, and 22.09%, co-inoculation with JYZ-SD5+Be significantly reduced by 15.58%, 26.38%, 53.66% and 30.39%(P < 0.05), respectively.At the same time, all inoculation treatments significantly reduced the activity of SOD, POD, CAT, APX and GR.4) Under salt stress, compared with uninoculated control, all inoculation treatments improved K+ content and reduced Na+ content, and thus reduced Na+/K+ ratio which was the highest in the single inoculation with Be and was significantly reduced by 23.84% and 26.58% in shoots and leaves of M.glyptostroboides (P < 0.05), respectively. Conclusion: This study showed that B.paramycoides JYZ-SD5 and S.commune Be can promote the growth of M.glyptostroboides, alleviate the damage of salt stress on M.glyptostroboides and improve the tolerance of M.glyptostroboides to salt stress by raising the plant water absorption and accumulation of osmotic regulation substances, maintaining the cell membrane stability, reducing the level of membrane lipid peroxidation and the accumulation of reactive oxygen species and antioxidant enzymes activity and reducing the Na+/K+ ratios.

Key words: Bacillus paramycoides JYZ-SD5, Schizophyllum commune Be, Metasequoia glyptostroboides, Salt stress, Salt tolerance

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