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林业科学 ›› 2011, Vol. 47 ›› Issue (2): 161-163.doi: 10.11707/j.1001-7488.20110222

• 研究简报 • 上一篇    下一篇

基于油茶组59万条EST序列的转录组学初步分析

陈英1, 江香梅2, 张露3, 张新叶4, 项东云5, 温强1,2, 江聪1, 王光萍1, 黄敏仁1, 徐立安1   

  1. 1. 南京林业大学 南京 210037;2. 江西省林业科学院 南昌 330032;3. 江西农业大学 南昌 330045;4. 湖北省林业科学研究院 武汉 430079;5. 广西林业科学研究院 南宁 530002
  • 收稿日期:2010-10-22 修回日期:2010-11-24 出版日期:2011-02-25 发布日期:2011-02-25
  • 通讯作者: 徐立安

Transcriptome Characterization for Camellia Sect. Oleifera Based on the 592 499 ESTs

Chen Ying1, Jiang Xiangmei2, Zhang Lu3, Zhang Xinye4, Xiang Dongyun5, Wen Qiang1,2, Jiang Cong1, Wang Guangping1, Huang Minren1, Xu Li'an1   

  1. 1. Nanjing Forestry University Nanjing 210037;2. Jiangxi Academy of Forestry Nanchang 330032;3. Jiangxi Agricultural University Nanchang 330045;4. Hubei Academy of Forestry Wuhan 430079;5. Guangxi Academy of Forestry Nanning 530002
  • Received:2010-10-22 Revised:2010-11-24 Online:2011-02-25 Published:2011-02-25

关键词: 油茶, 转录组, 454 GS FLX, 表达序列标签

Abstract:

To investigate gene diversity and expression pattern in Camellia Sect. Oleifera, 454 GS FLX platform and Titanium regent were used to produce expressed sequence tags (ESTs) from flower buds of 3 Camellia species. A total of 5 920 499 ESTs were generated. Clustering those sequences twice by Cap3 produced 15 733 contigs and 20 606 singlets in C. oleifera , 19 397 contigs and 26 882 singlets in C. chekiangoleosa , 14 779 contigs and 25 389 singlets in C. brevistyla . The GC gradients in 3 transcriptomes basically followed a normal distribution. In additional all contigs and singlets were functionally categorized into GO(Gene Ontology) categories respectively.

Key words: Camellia, transcriptome, 454 GS FLX, expressed sequence tags

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