纳米竹炭对杜仲提取物的吸附和缓释作用

doi:10.11707/j.1001-7488.20180615

林业科学 ›› 2018, Vol. 54 ›› Issue (6): 125-131.doi: 10.11707/j.1001-7488.20180615

纳米竹炭对杜仲提取物的吸附和缓释作用

曾朝彦¹, 李湘洲¹, 张胜¹, 黄丹^1,2

1. 中南林业科技大学材料科学与工程学院长沙 410004;
2. 湖南中医药大学中药粉体与创新药物省部共建国家重点实验室培育基地长沙 410208

收稿日期:2016-09-23 修回日期:2016-12-02 出版日期:2018-06-25 发布日期:2018-07-02
基金资助:
“十二五”国家科技支撑计划“竹藤资源化学利用”（2012BAD23B0302）。

Adsorption and Sustained Release of Eucommia ulmoides Extract on Nano-Bamboo Charcoal

Zeng Zhaoyan¹, Li Xiangzhou¹, Zhang Sheng¹, Huang Dan^1,2

1. College of Materials Science and Engineering of Central South University of Forestry and Technology Changsha 410004;
2. State Key Laboratory of Chinese Medicine Powder and Medicine Innovation in Hunan(Incubation), Hunan University of Chinese Medicine Changsha 410208

Received:2016-09-23 Revised:2016-12-02 Online:2018-06-25 Published:2018-07-02

摘要/Abstract

摘要： [目的]研究纳米竹炭（NBC）对杜仲提取物（EUE）的吸附和缓释效果，为纳米竹炭天然活性成分缓释制剂的开发和利用提供理论依据。[方法]先将EUE溶于蒸馏水中，再将NBC混悬于EUE溶液中，在37℃、pH 7.0条件下超声振荡36 min，采用紫外分光光度法检测NBC对EUE的吸附效果。将NBC-EUE给药体系在人工肠液中释放，在不同时间点取样测定其紫外吸光度值，同时补充消耗的人工肠液，计算不同时间点人工肠液中EUE的浓度，观察NBC对EUE的缓释效果，并用Higuchi方程拟合。通过SEM、BET、FTIR和TG等手段表征NBC吸附EUE前后的理化性质。采用MTT法检验不同时间点在人工肠液中释放出来的游离EUE对人直肠癌细胞（HCT116）的增殖抑制效果，并验证释放出来的EUE浓度是否与时间呈依懒性。[结果]NBC对EUE的饱和吸附时间为30 min，基于Langmuir吸附模型求得NBC对EUE的饱和吸附量为492.61 mg·g^-1。NBC在人工肠液中对EUE中天然活性成分的累积释放率为70.67%。BET结果显示，负载EUE前后NBC的比表面积分别为820.32 m²·g^-1和443.80 m²·g^-1，比表面积降低显著。SEM、FTIR和TG结果均表明，NBC成功负载了EUE。MTT结果显示，EUE释放液对人直肠癌细胞（HCT116）的抑制率为23.07%，EUE中天然活性成分在人工肠液中不同时间点释放出来的浓度与时间呈依懒性。[结论]一定时间内，NBC对EUE的吸附量随时间增加而增大，一定时间后达到饱和，在此区间可用Langmuir吸附模型描述。从体外释放动力学方面分析，Higuchi方程可较好描述NBC-EUE给药体系在人工肠液中的释放曲线，体外验证试验显示缓释液的浓度与时间呈正相关。本研究可为传统中药杜仲提取物提供一种新的纳米竹炭载药途径。

关键词: 纳米竹炭, 杜仲提取物, 吸附, 缓释

Abstract: [Objective] This study was carried out with aims to prepare a novel nano drug delivery system consisting of bamboo charcoal and Eucommia ulmoides, and to investigate its anticancer effects in vitro.[Method] The adsorption capacity of the nano-bamboo charcoal on Eucommia ulmoides extract was measured by Langmuir model, and the release experiment was carried out under intestinal fluid condition. Characteristic changes for the nano-bamboo charcoal drug delivery system with and without adsorption of Eucommia ulmoides were evaluated by scanning electron microscopy,Fourier transform infrared spectroscopy,thermogravimetric analysis,and specific surface area. In addition,the anticancer effect from this novel bamboo charcoal Eucommia ulmoides delivery system was evaluated against a human colon cancer cell line(HCT116).[Result] Nano-bamboo charcoal exhibited good adsorption capacity of the Eucommia ulmoides extract(up to 492.61 mg·g^-1 at 37℃. The cumulative release rate for the Eucommia ulmoides extract from this nano-bamboo charcoal delivery system was 70.67% and specific surface area of the nano-bamboo charcoal decreased from 820.32 m·g^-1 to 443.80 m²·g^-1 after the Eucommia ulmoides extract was loaded. Vitro anticancer study showed that the inhibition rate for the Eucommia ulmoides against HCT116 cancer cells was 23.07%,in this novel bamboo charcoal nano drug delivery system.[Conclusion] A novel strategy for the delivery of traditional Chinese medicine Eucommia ulmoides extract via bamboo charcoal nano drug delivery system was provided.

Key words: nano-bamboo charcoal(NBC), Eucommia ulmoides extract(EUE), adsorption, sustained release

中图分类号:

S567
R932

曾朝彦, 李湘洲, 张胜, 黄丹. 纳米竹炭对杜仲提取物的吸附和缓释作用[J]. 林业科学, 2018, 54(6): 125-131.

Zeng Zhaoyan, Li Xiangzhou, Zhang Sheng, Huang Dan. Adsorption and Sustained Release of Eucommia ulmoides Extract on Nano-Bamboo Charcoal[J]. Scientia Silvae Sinicae, 2018, 54(6): 125-131.

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纳米竹炭对杜仲提取物的吸附和缓释作用

Adsorption and Sustained Release of Eucommia ulmoides Extract on Nano-Bamboo Charcoal

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