欢迎访问林业科学,今天是

林业科学 ›› 2017, Vol. 53 ›› Issue (9): 123-132.doi: 10.11707/j.1001-7488.20170915

• 研究简报 • 上一篇    下一篇

杏李不同品种果实香气成分分析

李泰山, 韩卫娟, 杜改改, 刁松锋, 冯延芝, 杨绍彬, 岳华峰, 李芳东, 傅建敏   

  1. 国家林业局泡桐研究开发中心 中国林业科学研究院经济林研究开发中心 郑州 450003
  • 收稿日期:2016-08-12 修回日期:2017-08-01 出版日期:2017-09-25 发布日期:2017-10-24
  • 基金资助:
    国家林业局948项目"第二代杏李种间杂交新品种及栽培技术引进"(2014-4-20)。

Volatile Characteristics of Different Prunus domestica×armeniaca Cultivars Evaluated by HS-SPME with GC-MS

Li Taishan, Han Weijuan, Du Gaigai, Diao Songfeng, Feng Yanzhi, Yang Shaobin, Yue Huafeng, Li Fangdong, Fu Jianmin   

  1. China Paulownia Research Center, State Forestry Administration, Non-Timber Forest Research and Development Center, Chinese Academy of Forestry Zhengzhou 450003
  • Received:2016-08-12 Revised:2017-08-01 Online:2017-09-25 Published:2017-10-24

摘要: [目的]了解不同品种杏李果实香气成分及含量特征,为杏李果实评价和品种改良提供理论依据。[方法]以3-辛醇为内标,采用顶空固相微萃取-气质联用技术(HS-SPME-GC-MS)分别对杏李品种‘风味玫瑰’、‘味帝’、‘味王’、‘恐龙蛋’、‘味厚’和‘风味皇后’的果实香气进行定性和定量分析,并根据香气阈值确定杏李各品种的香气值和特征香气。[结果]6个品种的果实中共检测出6大类77种化合物,包括9种醇、16种醛、29种酯,9种内酯、4种萜和10种酮。6个杏李品种均含有己醇、甲基庚烯酮、苯乙酮、己醛、辛醛、5-羟甲基糠醛、苯甲醛、γ-癸内酯和芳樟醇,香气种类由多到少依次为:‘风味玫瑰’ > ‘味王’ > ‘恐龙蛋’ > ‘味帝’ > ‘风味皇后’ > ‘味厚’。杏李果实香气相对含量为37.22~277.05 μg·kg-1FW,平均含量为114.95 μg·kg-1FW,香气含量从高到低依次为‘味王’、‘风味玫瑰’、‘味厚’、‘味帝’、‘风味皇后’、‘恐龙蛋’。6个杏李品种中,醇类和内酯类含量最高的品种为‘风味玫瑰’,醛类和酮类含量最高的品种为‘味厚’,酯类含量最高的品种为‘味王’,萜类含量最高的品种为‘味帝’。‘风味玫瑰’的特征香气成分种类最多,有己醇、癸醛、乙酸丁酯、醋酸辛酯、己酸丁酯、己酸乙酯、γ-己内酯、γ-癸内酯和芳樟醇9种;‘味帝’的特征香气成分有己醛、壬醛、辛醛、乙酸丁酯和芳樟醇;‘味王’有己醇、乙酸丁酯、乙酸己酯、己酸丁酯、己酸乙酯、γ-癸内酯和芳樟醇7种特征香气,其中乙酸丁酯的香气值达到877.72 μg·kg-1FW;‘恐龙蛋’的特征香气成分有己醇、己醛和辛醛;‘味厚’的特征香气成分有己醇、己醛、辛醛和芳樟醇;‘风味皇后’的特征香气成分有己醛、辛醛、反式-2-壬醛和芳樟醇。在风味方面,‘风味玫瑰’和‘味王’果实风味以杏香味为主,并伴有少量李香味;‘味帝’以李香味为主,并伴有少量杏香味;‘恐龙蛋’、‘风味皇后’和‘味厚’具有明显的李香味特征。[结论]杏李果实香气资源丰富,不同品种间差异较大。‘风味玫瑰’和‘味王’属于酯香型品种,‘味帝’、‘风味皇后’和‘味厚’为萜醛混合香型品种,‘恐龙蛋’为醛醇混合香型品种。本研究筛选出‘风味玫瑰’和‘味王’2个高酯型品种,1个高萜类品种‘味帝’和2个高醛类品种‘风味皇后’和‘味厚’。

关键词: 杏李, 香气, 风味, 特征香气

Abstract: [Objective] Aroma components and contents in ripe fruits of six Prunus domestica×armeniaca cultivars were investigated to provide a theoretical basis for P. domestica×armeniaca quality evaluation and improvement.[Method] Volatiles of six P. domestica×armeniaca cultivars (‘Fengweimeigui’, ‘Weidi’, ‘Weiwang’, ‘Konglongdan’, ‘Weihou’, ‘Fengweihuanghou’) were qualitatively and quantitatively detected with 3-octanol as an internal standard by means of HS-SPME-GC, and odour activity values and characteristic components were also determined according to odour thresholds.[Result] A total of 77 compounds were identified, and they were divided into six categories including 9 alcohols, 16 aldehydes, 29 esters, 9 lactones, 4 terpenoids and 10 ketones. All the six P. domestica×armeniaca cultivars contained 1-hexanol, 5-Hepten-2-one, 6-methyl-, acetophenone, hexanal, octanal, 5-hydroxymethylfurfural, benzaldehyde, γ-decalactone and linalool. The number of aromatic volatile compounds in six varieties ranged from high to low was ‘Fengweimeigui’, ‘Weiwang’, ‘Konglongdan’, ‘Weidi’, ‘Fengweihuanghou’ and ‘Weihou’. The contents of aromatic components in six P. domestica×armeniaca cultivars ranged from 37.22 μg·kg-1 FW to 277.05 μg·kg-1 FW, and the average content was 114.95 μg·kg-1 FW. The contents of aromatic components ranged was in an order of ‘Weiwang’ > ‘Fengweimeigui’ > ‘Weihou’ > ‘Weidi’ > ‘Fengweihuanghou’ > ‘Konglongdan’. The contents of alcohols and lactones were the highest in ‘Fengweimeigui’, while aldehydes and ketones the highest in ‘Weihou’. ‘Weiwang’ contained the highest ester, whilst terpenoids in ‘Weidi’ had the highest content. Theresult determined characteristic components of aroma, of which ‘Fengweimeigui’ had the highest amount, including hexanol, decanal, acetic acid butyl ester, acetic acid octyl ester, butyl hexanoate, ethyl caproate, γ-caprolactone, γ-decalactone and linalool. The characteristic aromas in ‘Weidi’ were hexanal, heptyl, octyl aldehyde, butyl acetate and linalool. Characteristic components of aroma in ‘Weiwang’ were hexyl alcohol, butyl acetate, hexyl acetate, butyl hexanoate, ethyl caproate, gamma-decalactone and linalool.The odor activity value of butyl acetate in ‘Weiwang’ was 877.72 μg·kg-1 FW. The characteristic components in ‘Konglongdan’ were hexyl alcohol, caproaldehyde and octanal; hexyl alcohol, caproaldehyde, octanal and linalool in ‘Weihou’; while hexanalnal, octanal, trans-2-nonenal and linalool in ‘Fengweihuanghou’. ‘Fengweimeigui’ and ‘Weiwang’ smelt like a taste of apricot along with slight plum aroma, while it was opposite in ‘Weidi’; and other varieties including ‘Konglongdan’, ‘Fengweihuanghou’ and ‘Weihou’ had clear aroma of plum similarly.[Conclusion] Aromatic components and contents varied greatly among P. domestica×armeniaca cultivars. All the germplasms could be classified into three groups:1) ‘Fengweimeigui’ and ‘Weiwang’ with high content of ester; 2) ‘Weidi’, ‘Weihou’ and ‘Fengweihuanghou’ with high content of terpenoid and aldehyde, 3) ‘Konglongdan’ with high content of aldehyde and alcohols. Two cultivars, i.e. ‘Fengweimeigui’ and ‘Weiwang’, with high richness of esters were determined; ‘Weidi’ with high richness of terpenoids;‘Fengweihuanghou’ and ‘Weihou’ with high richness of aldehyde were chosen.

Key words: Prunus domestica×armeniaca, volatiles, flavor, characteristic components

中图分类号: