关键词: 741毛白杨, 双抗虫基因, 转化, 选择

Abstract:

Explants of hybrid poplar 741 [Populus alba L.×(P.davidiana Dode+P.simonii Carr.)×P.tomentosa Carr.] were transformed with Agrobacterium tumefaciens LBA4404 harboring the expression vector pBtiA containing two insect-resistant genes. Conditions for selection of poplar transforments, especially the critical concentrations of Kanamycin(Km') were determined. 80% of the transforments selected on rooting medium containing Km' gave out positive results by PCR detection. The gene-transformed plants preliminaries selected were given to insect feeding tests with Clostera anachoreta Fabricius and Lymantria dispar Linnacus.And the tested insects were divided into four groups, high, middle, low and control according to mortality. In the meantime, the insect mortality in fixed period and the effects of delaying the growth and development of insects were comparatively studied to provide the basis for selecting plants or clones, which significantly resist to insect. The results of detailed insect-bioassay and molecular biology analysis demonstrate that the two foreign genes have been integrated into the genomes of insect resistant plants. Transforments with normal phenotype, growth rate as that of the non-transformed control and high insect-resistant were selected and accounted for 38.1% of the transformed tested.

Key words: Hybrid poplar 741, Two insect-resistant genes, Transformed, Selection