中国沙棘种子的水引发技术及其抗性生理效应

doi:10.11707/j.1001-7488.20141205

摘要/Abstract

摘要：

研究不同温度和吸水时间对中国沙棘种子萌发的影响,确定种子引发最适条件; 干旱和盐胁迫条件下,通过对引发种子活力和幼苗形态指标的考察,探索引发对种子的抗性生理效应.结果表明: 1) 水引发沙棘种子的最适条件为10 ℃、避光条件下分批给种子加水,总水量为种子初始质量的55%,在引发0,3,6,12和21 h时分别加入总水量的23%,23%,25%,14%和15%,引发7天.2) 对中国沙棘4个产地(陕西、宁夏、甘肃、内蒙古)种子水引发效应进行验证,共测定种子可溶性糖、丙二醛(MDA)和脯氨酸3个指标,差异均极显著.3) 干旱和盐胁迫条件下,与对照比较,水引发显著提高种子发芽率、发芽指数,缩短种子平均发芽时间,提升临界萌发条件, 显著提高幼苗的苗高、根长和鲜质量.综合分析认为,水引发中国沙棘种子避免种子吸胀伤害和细胞膜结构被破坏,阻止内溶物的渗漏; 极显著提高引发种子可溶性糖含量,为种子萌发的启动提供保障; 极显著降低丙二醛含量,增强种子抗氧化系统的功能; 极显著提高脯氨酸含量,增强种子的抗逆应答反应.水引发技术成本低,效果显著,是增强种子活力、提高幼苗抗性的一条有效途径.

关键词: 水引发, 中国沙棘, 种子萌发, 种子活力, 幼苗抗性

Abstract:

Optimum conditions for seed priming of sea buckthorn (Hippophae rhamnoides subsp. sinensis) were determined by investigating the effects of different temperatures and water absorption rates on germination of sea buckthorn. Furthermore, the physiology effect of seed priming was investigated by evaluating the vigor, morphological indexes of seedlings under drought and salt stress conditions. Results showed that hydropriming condition was optimum at 10 ℃, providing water in batches to the seed in dark for seven days. The total volume of water used for hydropriming was 55% of the original mass of seeds. The 23%, 23%, 25%, 14% and 15% of the total water volume were added in 0, 3, 6, 12, 21 h of hydropriming, respectively. The hydropriming effects of sea buckthorn seeds were evaluated by measuring the content of soluble sugar, MDA and proline in seeds from 4 different provinces (Shaanxi, Ningxia, Gansu, Inner Mongolia). Hydropriming seeds of sea buckthorn significantly improved seed germination rate and germination index. The hydropriming also reduced mean germination time and increased seedling height, root length, fresh weight, and enhanced critical germination conditions under drought and salt stress conditions as compared to control. Comprehensive analysis indicated that hydropriming could control seed imbibitions and prevent cell membrane structure from being destroyed, thereby preventing the leakage of soluble substances. This action significantly improved seed soluble sugar content and hence provided a guarantee for germination. It also decreased MDA content and enhanced the function of seed antioxidant system. The hydropriming significantly increased proline content, thereby enhancing seed resilience. Hydropriming technology is inexpensive and it may be an effective way of enhancing seed vigor and seedling resistance.

Key words: hydropriming, sea buckthorn, seed germination, seed vigor, seedling resistance

中图分类号:

S718.43

孙妙, 杨周婷, 张存莉, 魏安智, 史伟. 中国沙棘种子的水引发技术及其抗性生理效应[J]. 林业科学, 2014, 50(12): 32-39.

Sun Miao, Yang Zhouting, Zhang Cunli, Wei Anzhi, Shi Wei. Hydro-Priming Technique and Its Resistance Physiology Effect for Sea Buckthorn Seed[J]. Scientia Silvae Sinicae, 2014, 50(12): 32-39.

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