基于分子可操作分类单元的北京地区杨树内生葡萄座腔菌属真菌多样性

doi:10.11707/j.1001-7488.20140117

林业科学 ›› 2014, Vol. 50 ›› Issue (1): 109-115.doi: 10.11707/j.1001-7488.20140117

基于分子可操作分类单元的北京地区杨树内生葡萄座腔菌属真菌多样性

李如华, 严东辉, 冯小慧, 孙晓明

中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室北京 100091

收稿日期:2013-07-09 修回日期:2013-10-14 出版日期:2014-01-25 发布日期:2014-01-25
基金资助:
“十二五”国家高新技术研究和发展计划项目（863）（2012AA101501）；引进国际先进林业科学技术计划项目（2013-4-10）。

Diversity of Botryosphaeria spp., as Endophytes in Poplars in Beijing, Based on Molecular Operational Taxonomic Units

Li Ruhua, Yan Donghui, Feng Xiaohui, Sun Xiaoming

Key Laboratory of Forest Protection of State Forestry Administration Research Institute of Forest Ecology, Environment and Protection, CAF Beijing 100091

Received:2013-07-09 Revised:2013-10-14 Online:2014-01-25 Published:2014-01-25
Contact: 严东辉

摘要/Abstract

摘要：

树木内生真菌的多样性是树木健康和树木与环境互作的基础，基于分子可操作分类单元的DNA条形码在揭示微生物多样性上起着重要作用。依据DNA条形码标准和技术，经由来自公共核酸数据库（NCBI）中葡萄座腔菌属及其相关属的ITS序列有效化以及对杨树皮层组织分离培养真菌ITS序列扩增和亲缘关系分析发现，该属真菌是常见的杨树内生真菌，在北京地区的杨树上存在葡萄座腔菌、红葡萄座腔菌和小葡萄座腔菌3个种群，葡萄座腔菌为优势种群，每个种群内也存在遗传分化。

关键词: 内生真菌, Botryosphaeria, DNA 条形码, 分类, 多样性

Abstract:

Diversity of endophytic fungi in trees is a key to tree health and interaction between trees and environment. Operational taxonomic units based on molecular DNA barconding an important role in disclosing microbiological diversity. Based on criteria and techniques of DNA barcoding approaches, a validation database on ITS sequences of Botryosphaeria genus and its related fungi downloaded from public nucleotide databases in NCBI, was constructed for determining the strains from endophytes in poplars in Beijing area, along with their ITS amplification and phylogenetic relationship analysis. The results showed that there were three populations of Botryosphaeria species parasitic in poplars as endophytes,B. dothidea, B. rhodina and B. parva. Strains of each population had genetica diversity. The approaches and results, presented in this paper,would contribute to a comprehensive understanding endophyte diversity in poplars and promoting researches on relationships between poplar hosts and Botryosphaeria pathogen as well as poplar canker fungal pathagenesis and control.

Key words: endophyte, Botryosphaeria, DNA barcoding, classification, diversity

中图分类号:

S718.81

李如华, 严东辉, 冯小慧, 孙晓明. 基于分子可操作分类单元的北京地区杨树内生葡萄座腔菌属真菌多样性[J]. 林业科学, 2014, 50(1): 109-115.

Li Ruhua, Yan Donghui, Feng Xiaohui, Sun Xiaoming. Diversity of Botryosphaeria spp., as Endophytes in Poplars in Beijing, Based on Molecular Operational Taxonomic Units[J]. Scientia Silvae Sinicae, 2014, 50(1): 109-115.

参考文献

姜自如, 张刚龙, 曹支敏, 等.2012.陕西省杨树溃疡病菌地理种群研究. 西北林学院学报, 27(2): 102-108.
(Jiang Z R, Zhang G L, Cao Z M, et al. 2012. Geographical species of poplar canker' pathogen in Shaanxi Province. Journal of Northwest Forestry University, 27(2): 102-108. [in Chinese] )
王金利, 贺伟, 秦国夫, 等. 2007.树木溃疡病重要病原葡萄座腔菌属、种及其无性型研究. 林业科学研究, 20(1): 21-28.
(Wang J L, He W, Qin G F, et al. 2007. Study on the anamorph and species of botryosphaeria,the important pathogen of tree's canker.Forest Research, 20(1): 21-28. [in Chinese] )
张星耀, 骆有庆.2003.中国森林重大生物灾害. 北京: 中国林业出版社.
(Zhang X Y, Luo Y Q. 2003. Major forest diseases and insect pests in China. Beijing: China Forestry Publishing House. [in Chinese] )
Abreo E, Martinez S, Bettucci L, et al. 2013.Characterization of Botryosphaeriaceae species associated with grapevines in Uruguay. Australasian Plant Pathol, 42(3): 241-249.
Álvarez-Loayza P, White J J, Torres M, et al. 2011. Light converts endosymbiotic fungus to pathogen, influencing seedling survival and niche-space filling of a common tropical tree, Iriartea deltoidea. PLoS ONE, 6(1): e16386.
Alves A, Crous P W, Correia A, et al.2008.Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity, 28: 1-13.
Buée M, Reich M, Murat C, et al.2009.454 pyrosequencing analyses of forest soils reveal an unexpectedly high fungal diversity. New Phytologist, 184(2): 449-456.
Begerow D, Nilsson H, Unterseher M, et al.2010.Current state and perspectives of fungal DNA barcoding and rapid identification procedures. Appl Microbiol Biotechnol, 87(1): 99-108.
Cai Lei, Giraud T, Zhang Ning, et al. 2011.The evolution of species concepts and species recognition criteria in plant pathogenic fungi. Fungal Diversity, 50: 121-133.
Crous P W, Slippers B, Wingfield M J, et al.2006. Phylogenetic lineages in the Botryosphaeriaceae. Studies in Mycology, 55(1): 235-253.
Hibbett D S, Taylor J W. 2013. Fungal systematic: is a new age of enlightenment at hand? Nature Reviews Microbiology, 11: 129-133.
McDonald V, Eskalen A.2011.Botryosphaeriaceae species associated with avocado branch cankers in California. Plant Disease, 95(11): 1465-1473.
Schoch C L, Seifert K A, Huhndorf S, et al.2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA, 109(16), 6241-6246.
Slippers B, Crous P W, Denman S, et al. 2004.Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea. Mycologia, 96(1): 83-101.
Slippers B, Wingfield J M.2007.Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact. Fungal Biology Reviews, 21(2-3): 90-106.
Smith H, Wingfield M J, Petrini O. 1996.Botryosphaeria dothidea endophytic in Eucalyptus grandis and Eucalyptus nitens in South Africa. Forest Ecology and Management, 89(1-3): 189-195.
Vrlstad T. 2011.ITS, OTUs and beyond—fungal hyperdiversity calls for supplementary solutions. Molecular Ecology, 20(14): 2873-2875.
White T J, Bruns T, Lee S, et al. 1990.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics//PCR Protocols: A Guide to Methods and Applications. San Diego: Academic Press, 315-322.

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基于分子可操作分类单元的北京地区杨树内生葡萄座腔菌属真菌多样性

Diversity of Botryosphaeria spp., as Endophytes in Poplars in Beijing, Based on Molecular Operational Taxonomic Units

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