甜樱桃矮化砧‘吉塞拉6号’扦插过程中氧化酶活性和碳氮含量变化

林业科学 ›› 2013, Vol. 49 ›› Issue (9): 172-177.

甜樱桃矮化砧‘吉塞拉6号’扦插过程中氧化酶活性和碳氮含量变化

魏海蓉¹, 陈新¹, 宗晓娟¹, 王甲威¹, 张庆霞², 张道辉¹, 刘庆忠¹

1. 山东省果树研究所山东省果树生物技术育种重点实验室泰安 271000;
2. 陇东学院农林科技学院庆阳 745000

收稿日期:2013-06-16 修回日期:2013-07-08 出版日期:2013-09-25 发布日期:2013-09-19
通讯作者: 刘庆忠
基金资助:
公益性行业(农业)科研专项(200903019);国家国际科技合作专项(2012DFR30700);山东省农业良种工程项目。

Fluctuations of Oxidase Activities and Carbon and Nitrogen Content during the Rooting Process of Sweet Cherry Dwarf Rootstock ‘Gisela 6’ Softwood Cuttings

Wei Hairong¹, Chen Xin¹, Zong Xiaojuan¹, Wang Jiawei¹, Zhang Qingxia², Zhang Daohui¹, Liu Qingzhong¹

1. Shandong Institute of Pomology Key Laboratory for Fruit Biotechnology Breeding of Shandong Tai'an 271000;
2. College of Agroforestry Sci-Tech, Longdong University Qingyang 745000

Received:2013-06-16 Revised:2013-07-08 Online:2013-09-25 Published:2013-09-19

摘要/Abstract

关键词: &lsquo, 吉塞拉6号&rsquo, 嫩枝扦插, IBA, 不定根发生, 氧化酶, 营养物质

Abstract:

In this paper, we investigated the rooting characteristics of the softwood cutting of 'Gisela 6'(Prunus cerasus × P. canescens)and the changes in activity of peroxidase (POD), polyphenoloxidase (PPO), indoleacetic acid oxidase (IAAO), total soluble sugar content, total nitrogen content and C/N ratio during the rooting. The results showed that:The rooting percentage, the root number and the average root length increased significantly when treated by IBA. The treatment of 1 000 mg·L^-1 IBA had the best effect on the rooting. The rooting percentage, the root number and the average root length were 81.2%, 10.5 and 2.95 cm, respectively. Compared with the control, the rooting percentage, the root number and the average root length increased by 133.3%, 262.1% and 45.3%, respectively. The activity of PPO, POD and IAAO, total soluble sugar content and C/N ratio changed regularly during the rooting. The activity of PPO and POD increased during the adventitious root induction and initiation, reached to a peak level on day 18, and then decreased during the root elongation period. However, the activity of IAAO decreased during the induction and initiation phase and increased slowly in the root elongation phase. The total soluble sugar content and C/N ratio increased during the induction phase, and then decreased in the following days. The total nitrogen content presented a continued decrease in the whole rooting process. The activity of PPO and POD enhanced significantly and changed more sharply during the rooting period after treatment with IBA. The IBA treatment also enhanced the total soluble sugar content and C/N ratio, but the total nitrogen content did not changed significantly. These results indicated that the activity of PPO, POD and IAAO, the total soluble sugar content and C/N ratio were related to the development of adventitious root closely. IBA increased rooting activities of the Gisela 6 softwood mainly owing to the increase of the activity of PPO and POD, the total soluble sugar content and C/N ratio and the reduction of the IAAO activity.

Key words: ‘Gisela 6’(Prunus cerasus×, P. canescens), softwood cutting, IBA, adventitious root development, oxidases, nutrients

中图分类号:

S723.13

魏海蓉, 陈新, 宗晓娟, 王甲威, 张庆霞, 张道辉, 刘庆忠. 甜樱桃矮化砧‘吉塞拉6号’扦插过程中氧化酶活性和碳氮含量变化[J]. 林业科学, 2013, 49(9): 172-177.

Wei Hairong, Chen Xin, Zong Xiaojuan, Wang Jiawei, Zhang Qingxia, Zhang Daohui, Liu Qingzhong. Fluctuations of Oxidase Activities and Carbon and Nitrogen Content during the Rooting Process of Sweet Cherry Dwarf Rootstock ‘Gisela 6’ Softwood Cuttings[J]. Scientia Silvae Sinicae, 2013, 49(9): 172-177.

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