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林业科学 ›› 2013, Vol. 49 ›› Issue (4): 152-156.doi: 10.11707/j.1001-7488.20130423

• 研究简报 • 上一篇    下一篇

α-蒎烯对大麦虫体内解毒酶活性的影响

田雨浓1, 马伟2, 韦庆慧1, 韩旭洋1, 罗帅1, 陈旭日1, 邱本军1, 马玲1   

  1. 1. 东北林业大学 哈尔滨 150040;2. 黑龙江中医药大学 哈尔滨 150040
  • 收稿日期:2012-07-05 修回日期:2013-02-16 出版日期:2013-04-25 发布日期:2013-04-25
  • 通讯作者: 马玲

Effects of α-pinene Fumigation on Detoxifying Enzymes Activities of Zophobas morio

Tian Yunong1, Ma Wei2, Wei Qinghui1, Han Xuyang1, Luo Shuai1, Chen Xuri1, Qiu Benjun1, Ma Ling1   

  1. 1. Northeast Forestry University Harbin 150040;2. Chinese Medicine, Heilongjiang University Harbin 150040
  • Received:2012-07-05 Revised:2013-02-16 Online:2013-04-25 Published:2013-04-25

关键词: α-蒎烯, 大麦虫, 急性毒性, 解毒酶

Abstract: In order to study the insecticidal activity and toxicity mechanism of α-pinene(a plant volatile organic compound), we tested the acute toxicity of α-pinene against 4th instar larvae of Zophobas morio in a gas-fumigation system, and investigated the activities of carboxylesterase (CarE), acid phosphatase (ACP), alkaline phosphatase (ALP) and the glutathione S-transfer (GSTs)in the larvae at different fumigation time. The results showed that the acute toxicity LC50 and LC20 values of α-pinene against the 4th instar larvae in 48 h were 21.435 and 12.140 μg ·L-1, respectively. Under the fumigation with LC50 and LC20 concentrations (21.435 and 12.140 μg ·L-1) of α-pinene, the activity of CarE was significantly inhibited. The activities of ALP and GSTs first increased but subsequently reduced. Under the fumigation at LC20 concentration of α-pinene, the activities of ACP was increase. Under the fumigation at LC20 concentrations of α-pinene, the ACP activity showed first increased but subsequently reduced. It could be inferred from the above results that α-pinene had a high biological activity on Z. morio, which could effectively interference the detoxifying enzymes, disrupt the normal physiological metabolism and show high toxicity effect on the insect.

Key words: α-pinene, Zophobas morio, acute toxicity, detoxifying enzymes

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