林业科学 ›› 2013, Vol. 49 ›› Issue (4): 152-156.doi: 10.11707/j.1001-7488.20130423
• 研究简报 • 上一篇 下一篇
田雨浓1, 马伟2, 韦庆慧1, 韩旭洋1, 罗帅1, 陈旭日1, 邱本军1, 马玲1
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Tian Yunong1, Ma Wei2, Wei Qinghui1, Han Xuyang1, Luo Shuai1, Chen Xuri1, Qiu Benjun1, Ma Ling1
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关键词: α-蒎烯, 大麦虫, 急性毒性, 解毒酶
Abstract: In order to study the insecticidal activity and toxicity mechanism of α-pinene(a plant volatile organic compound), we tested the acute toxicity of α-pinene against 4th instar larvae of Zophobas morio in a gas-fumigation system, and investigated the activities of carboxylesterase (CarE), acid phosphatase (ACP), alkaline phosphatase (ALP) and the glutathione S-transfer (GSTs)in the larvae at different fumigation time. The results showed that the acute toxicity LC50 and LC20 values of α-pinene against the 4th instar larvae in 48 h were 21.435 and 12.140 μg ·L-1, respectively. Under the fumigation with LC50 and LC20 concentrations (21.435 and 12.140 μg ·L-1) of α-pinene, the activity of CarE was significantly inhibited. The activities of ALP and GSTs first increased but subsequently reduced. Under the fumigation at LC20 concentration of α-pinene, the activities of ACP was increase. Under the fumigation at LC20 concentrations of α-pinene, the ACP activity showed first increased but subsequently reduced. It could be inferred from the above results that α-pinene had a high biological activity on Z. morio, which could effectively interference the detoxifying enzymes, disrupt the normal physiological metabolism and show high toxicity effect on the insect.
Key words: α-pinene, Zophobas morio, acute toxicity, detoxifying enzymes
中图分类号:
S718.7
田雨浓;马伟;韦庆慧;韩旭洋;罗帅;陈旭日;邱本军;马玲. α-蒎烯对大麦虫体内解毒酶活性的影响[J]. 林业科学, 2013, 49(4): 152-156.
Tian Yunong;Ma Wei;Wei Qinghui;Han Xuyang;Luo Shuai;Chen Xuri;Qiu Benjun;Ma Ling. Effects of α-pinene Fumigation on Detoxifying Enzymes Activities of Zophobas morio[J]. Scientia Silvae Sinicae, 2013, 49(4): 152-156.
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