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林业科学 ›› 2007, Vol. 43 ›› Issue (08): 65-70.doi: 10.11707/j.1001-7488.20070811

• 论文及研究报告 • 上一篇    下一篇

河朔荛花对山楂叶螨作用机制的初步研究

曹挥1,2 王有年1 张铁强1 赵莉蔺3 刘素琪2 师光禄1   

  1. 1.北京市农业应用新技术重点实验室,北京102206;2.山西农业大学农学院太谷030801;3.中国科学院动物研究所农业虫鼠害综合治理国家重点实验室,北京100080
  • 收稿日期:2005-11-09 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

Acarocidal Actions of Wikstroemiae chamedaphne against Tetranychus viennensis

Cao Hui1,2,Wang Younian1,Zhang Tieqiang1,Zhao Lilin3,Liu Suqi2,Shi Guanglu1   

  1. 1.Key Laboratory of New Technology of Agricultural Application of Beijing Beijing 102206;2.College of Agriculture,Shanxi Agricultural University Taigu 030801;3.State Key Laboratory of Integrated Management of Insects and Rodents Institute of Zoology,Chinese Academy of Sciences Beijing 100080
  • Received:2005-11-09 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要:

对河朔荛花活性成分最佳流分WCME-7和WCME-11处理后的雌成螨进行症状观察,初步推测它们可能通过影响山楂叶螨的神经传导和生殖、消化系统而导致雌成螨死亡。其中WCME-11击倒作用迅速,从12h开始山楂叶螨就进入了大量死亡期,具有比WCME-7更为强烈的症状表现和更全方位的作用方式。WCME-11能引起成螨体内的生理生化代谢,解毒酶类的活性被激活,Ca2+-ATP酶和乙酰胆碱酯酶活性被强烈抑制。

关键词: 河朔荛花, 山楂叶螨, 作用机制

Abstract:

Wikstroemiae chamedaphne has good acarocidal activity against Tetranychus viennensis,a serious pest of many crops including apple trees in China.Its active constitutes,WCME-7 and WCME-11,were separated and purified by column chromatograghy.The symptomal observiation showed that they had many actions,such as stomach poison,controlling development,controlling reproducing and so on.They maybe affect not only the nerve transmition but also digest,growing system.In contrast,WCME-11 showed more strong symptom phenomenon with more all-directions function method,knocked down more quickly.From 12 h,the mites began to enter death period on large.Function mechanism of WCME-11 was studied in the experiment.The results indicate that the WCME-11 could cause the fast reaction of the enzymes inside the mites.Before 8 h, the enzymes were changed.WCME-11 could cause mite to counteract poison by GSTS and CarE,Ca2+-ATP enzyme and AchE have been restrained strongly.

Key words: Wikstroemiae chamedaphne, Tetranychus viennensis, function mechanism