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林业科学 ›› 2001, Vol. 37 ›› Issue (5): 41-48.doi: 10.11707/j.1001-7488.20010508

• 论文及研究报告 • 上一篇    下一篇

云南红豆杉天然种群遗传多样性研究

陈少瑜 吴丽圆 李江文 项伟 周云   

  1. 云南省林业科学院,昆明650204
  • 收稿日期:2000-01-10 修回日期:1900-01-01 出版日期:2001-09-25 发布日期:2001-09-25

STUDY ON GENETIC DIVERSITY OF NATURAL POPULATIONS OF TAXUS YUNNANENSIS

Chen Shaoyu,Wu Liyuan,Li Jiangwen,Xiang Wei,Zhou Yun   

  1. Yunnan Academy of Forest Science Kunming 650204
  • Received:2000-01-10 Revised:1900-01-01 Online:2001-09-25 Published:2001-09-25

摘要:

采用垂直板式聚丙烯酰胺凝胶电泳,检测了云南红豆杉(Taxus yunnanensis)雌配子体的5种等位酶,并以10个基因位点编码的5个酶系统,分析了滇西北3个云南红豆杉天然种群的遗传多样性与遗传分化。结果表明,群体的多态位点比例是0.77;群体的平均杂合性观察值是0.302;预期值为0.3320;每个基因位点发现的等位基因数是2.05;有效等位基因平均数是1.466。对10个基因位点的基因多样性测定表明,种群间的分化占14.66%,总的基因多样性约85%产生于种群内。群体间的平均遗传距离是0.118。

关键词: 云南红豆杉, 等位酶, 种群, 遗传多样性, 遗传分化

Abstract:

Vertical slab polyacrylamide gel electrophoresis was used to examine inheritance of five isoenzymes in megagametophytes of Taxus Yunnanensis.Genetic diversity and genetic differentiation in three natural populations of Taxus Yunnanensis in Northwestern Yunnan were analyzed at ten loci coding for five enzyme systems.The percentage of loci polymorphism was 0.77.The average observed heterozygosity of all populations was 0.302,and the average expected heterozygosity was 0.320.The average number of alleles detected per locus was 2.05.The effective number of alleles per locus was 1.466.Measurement of gene diversity for ten loci showed that interpopulation genetic differentiation was 14.66%,and about 85% of the total gene diversity resided within population.The mean genetic distance was 0.118.

Key words: Taxus yunnanensis, Isoenzyme, Population, Genetic diversity, Genetic differentiation