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林业科学 ›› 2006, Vol. 42 ›› Issue (9): 29-33.doi: 10.11707/j.1001-7488.20060906

• 论文及研究报告 • 上一篇    下一篇

杉木成熟合子胚器官发生和体胚发生

席梦利 施季森   

  1. 南京林业大学国家林业局林木遗传和基因工程重点实验室,南京210037
  • 收稿日期:2005-07-26 修回日期:1900-01-01 出版日期:2006-09-25 发布日期:2006-09-25

Organogenesis and Somatic Embryogenesis from Mature Zygotic Embryos of Cunninghamia lanceolata

Xi Mengli,Shi Jisen   

  1. Key Laboratory of Forest Tree Genetics and Gene Engineering, State Forestry Administration Nanjing Forestry University Nanjing 210037
  • Received:2005-07-26 Revised:1900-01-01 Online:2006-09-25 Published:2006-09-25

摘要:

以杉木成熟合子胚为起始外植体,研究基本培养基、不同种类的细胞分裂素及生长素对杉木器官发生和体胚发生及不定芽生根的影响,并用石蜡切片法观察不定芽和体胚的发生和发育过程。结果表明:DCR+6-BA1.0mg·L-1+TDZ0.002 mg·L-1+NAA0.1 mg·L-1对成熟合子胚诱导不定芽最有效;DCR+6-BA 1.0 mg·L-1+TDZ 0.003mg·L-1+ NAA 0.1 mg·L-1是成熟合子胚诱导体胚的最佳培养基;不定芽在DCR基本培养基中可有效伸长;1/4MS+IBA 0.2 mg·L-1+NAA 0.1 mg·L-1对不定芽生根最有效。石蜡切片观察表明:不定芽有2种发生方式,一种是表皮及表皮下的3~4层细胞分生组织化发育而成,另一种是外植体内部的小维管束脱分化发育而成。2种发生方式均为直接器官发生途径。

关键词: 杉木, 成熟合子胚, 器官发生, 体胚发生

Abstract:

Using mature zygotic embryos as initial explants, the effect factors including basal medium and plant hormone on organogenesis, somatic embryogenesis and adventitious buds induction roots were investigated respectively. The origin and development of somatic embryos and adventitious buds were observed through the paraffin method. The results indicated that the medium of DCR+6-BA 1.0 mg·L-1 +TDZ 0.002 mg·L-1 +NAA 0.1 mg·L-1 was optimal for mature zygotic embryos to induce adventitious buds. The optimal medium for mature zygotic embryos to induce somatic embryos was DCR+6-BA 1.0 mg·L-1+TDZ 0.003 mg·L-1 + NAA 0.1 mg·L-1 . Adventitious buds can elongate effectively on the DCR medium without plant hormone. 1/4MS+IBA 0.2 mg·L-1 +NAA 0.1 mg·L-1 was the optimal medium for adventitious buds to root. Histological observation indicated that there were two ways in which adventitious buds originate. The first way of origination was from meristematic tissue developed from epiderm cells and three or four liners under epiderm. The other was that the little vascular bundle dedifferentiated into meristematic tissue, which in turn developed into adventitious buds. Both of the two ways were direct organogenesis.

Key words: Cunninghamia lanceolata, mature zygotic embryos, organogenesis, somatic embryogenesis