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林业科学 ›› 2006, Vol. 42 ›› Issue (10): 80-84.doi: 10.11707/j.1001-7488.20061015

• 论文及研究报告 • 上一篇    下一篇

鞭角华扁叶蜂体内多胺的动态

王满囷1 张志春1 李娟1 王楠1 薛东1 张国安1 李周直2   

  1. 1.华中农业大学植物科技学院,武汉430070;2.南京林业大学,南京210037
  • 收稿日期:2004-11-19 修回日期:1900-01-01 出版日期:2006-10-25 发布日期:2006-10-25

Changes of Polyamines of Chinolyda flagellicornis

Wang Manqun1,Zhang Zhichun1,Li Juan1,Wang Nan1,Xue Dong1,Zhang Guoan1,Li Zhouzhi2   

  1. 1.College of Plant Science and Technology,Huazhong Agriculture University Wuhan 430070;2.Nanjing Forestry University Nanjing 210037
  • Received:2004-11-19 Revised:1900-01-01 Online:2006-10-25 Published:2006-10-25

摘要:

用紫外荧光法测定鞭角华扁叶蜂末龄幼虫及滞育预蛹血淋巴中多胺的动态。结果表明鞭角华扁叶蜂血淋巴中含有8种多胺,其中腐胺(Put)、亚精胺(Spd)、精胺(Spm)、尸胺(Cad)为普遍存在的多胺,其发育的不同阶段,多胺的种类和含量均发生变化;在滞育预蛹的血淋巴中Spm一直保持较高的含量,并在整个滞育期间存在2个峰值;在滞育的前5个月中血淋巴中不存在Spd,之后Spd突然出现,且含量迅速升高;Cad的代谢较平稳,在化蛹前稍有下降;Put的含量除在滞育后3个月左右有较大的峰值外,其余阶段的含量均较高,并在化蛹前有升高的趋势。总之,多胺可能参与鞭角华扁叶蜂预蛹滞育过程。

关键词: 鞭角华扁叶蜂, 滞育, 多胺

Abstract:

The dynamics of haemolymph ployamines of the last instar and diapausing prepupa of the sawfly Chinolyda flagellicornis were determined by fluorescence spectrophotometer. The results showed that there were eight kinds of polyamines in the haemolymph of the sawfly,the putrescine(Put)、spermidine(Spd)、spermine(Spm) and cadaverine(Cad) were common polyamines,and the titres and kinds of polyamines were varied with the stages of development. The tires of Spm of diapausing prepupa maintained in a higher level,and formed two separated Spm concentration peak values. The Spd could not be detected in the first five months of diapausing prepua,but it had a higher level in the next development. The tires of Cad were going up a little before pupation,while the titres of Put were higher during the whole development of diapausing prepupa,formed one peak values in the times of 90 days of development,and going up sharply before pupation. It seemed that the polyamines are involved in regulating the diapause of C. flagellicornis.

Key words: Chinolyda flagellicornis, diapause, polyamines