关键词: 石楠, 茎尖, 玻璃化, 超低温

Abstract:

A procedure was studied on cryopreservation of Photinia serrulata shoot-tips in vitro by vitrification. The excised shoot-tips which were cultured for 30 d, 1.5～2.0 mm long, were loaded with 60% PVS₂ for 40 min at room temperature in effendoff tube, then were exposed to PVS₂ at 0 ℃ for 50 min. Followed by changing the solution with fresh PVS₂, the tubes were immersed into LN₂ directly. After 24 h storage, the effendoff tubes were rapidly thawed in the water bath at 40 ℃, the shoot-tips were washed twice with 1.2 mol·L^{-1 sucrose solution for 10 min and transferred onto MS medium supplemented with 6-BA 2.0 mg·mL-1. The cultures were kept in dark for one week prior to exposure to the light. Survival rate of shoot-tips was 93.33% by TTC examination, and regeneration rate reached 41.67%.}

Key words: Photinia serrulata, shoot-tips, vitrification, cryopreservation