林业科学 ›› 2007, Vol. 43 ›› Issue (05): 111-115.doi: 10.11707/j.1001-7488.20070519
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赵建萍1 柏新富1 蒋小满1 张萍1 BiKehua2
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Zhao Jianping1,Bai Xinfu1,Jiang Xiaoman1,Zhang Ping1,Bi Kehua2
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关键词: 北高丛越桔, 芽器官, 离体培养, 快繁
Abstract:
The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the explants were sterilized with alcohol,sap nation of cresol and HgCl2,and then cultured on WPM medium containing ZT 3.0 mg·L-1 and 3% sucrose;2)the medium for clump bud induction was WPM + ZT 2.0 mg·L-1 + 3% sugar;3)the medium for robust shoot was WPM + ZT 0~1.0 mg·L-1 + 3% sugar;4)for adventitious root induction from shoots,the medium was 1/2 WPM liquid nutritions containing IBA 1.0~5.0 mg·L-1 and 2% sugar,in which either vermiculite,filter paper or sterilized cotton were added,and shoots were cultured under a photoperiod of 12 h illumination and 12 h darkness alternatively after 14 days in darkness;5)the medium for transplanting was turfy-soil and mosses(V∶V= 1∶1).
Key words: Vaccinium corymbosum, sprout, culture in vitro, rapid propagation
赵建萍 柏新富 蒋小满 张萍 BiKehua. 北高丛越桔芽器官离体培养与快繁体系的建立[J]. 林业科学, 2007, 43(05): 111-115.
Zhao Jianping;Bai Xinfu;Jiang Xiaoman;Zhang Ping;Bi Kehua. Rapid Propagation of Plantlets from Sprout of Vaccinium corymbosum in Vitro[J]. Scientia Silvae Sinicae, 2007, 43(05): 111-115.
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