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›› 1985, Vol. 21 ›› Issue (4): 375-382.

• 论文及研究报告 • 上一篇    下一篇

外生菌根菌对油松苗木生物产量的影响

王昌温 罗晓芳 雷增普   

  1. 北京林学院
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1985-11-25 发布日期:1985-11-25

THE EFFECT OF ECTOMYCORRHIZAL FUNGI ON BIOMASS PRODUCTION OF PINUS TABULAEFORMIS SEEDLINGS

Wang Changwen,Luo Xiaofang,Lei Zengpu   

  1. Beijing College of Forestry
  • Received:1900-01-01 Revised:1900-01-01 Online:1985-11-25 Published:1985-11-25

摘要: 本试验验证了菌根化能促进油松苗木早期生长,对其生物产量有明显提高。优良菌种可使苗木干物重提高40%。试验筛选出Boletus sp.(林)、Suillusgrevillei(林)、Pisolithus tinctorius及“火炬松”、“欧洲赤松”等五个菌种在促进苗木生长方面有良好效果。经温室生物测定,不论是从菌根组织还是直接从子实体上分离到的菌种,接种后均能与油松根很好地形成菌根。用生长优良的固体纯培养物接种后,油松苗形成菌根的能力很强,多数产生茂盛的珊瑚状菌根组织,苗木的菌根化程度与其对生长的促进作用(包括干物重、高及粗生长)基本上呈正相关。在碱性及接近重碱性土壤中苗木均形成了菌根,并较好地起到促进生长作用,扩大了菌根菌抗逆性的筛选内容。试验筛选出适合菌根菌生长的培养基以及用于接种的纯培养物的载体。

Abstract: The authors'experiments proved that ectomycorrhizae may improve the early growth of Pinus tabulaeformis.It obviously stimulates the biomass production of pine seedlings.The approximate percentage of increase is 40%. Among the mycorrhizal fungi used in our experiments,five were selected as good ones which are capable of forming ectomycorrhizae and stimulating growth of P.tabulaeformis seedlings,i.e,Boletus sp.,suillus grevillei,Pisolithus tinctorius,and strains obtained from the ectomycorrhizal association of loblolly pine and scotch pine trees. Biological analysis in greenhouse studies shows that all species mentioned above,whether collected from mycorrhizae association or from mature fruitbodies of fungi,can easily from ectomycorrhizae with P.tabulaeformis. The results of experiments show that by inoculum of solid pure culture,mycorrhizal fungi on roots have a great capacity to establish ectomycorrhizae of coral type.The stimulation of seedling growth (including measurements of height,thickness and biomass)is proportional to the development of mycorrhizae on it. The fact that acid soil conditions are needed for the formation of ectomycorrhizae is well documented throughout the world.Usually ectomycorrhizae develops either weakly or not at all basic soil.But in the authors' experiments mycorrhizae are formed both in nursery soil (pH8.6) and in greenhouse soil (with alkalinity of up to 8.9),and can improve the growth of seedlings well.It has been proved by these authors that even under harsh soil conditions suitable strains can survive and grow significantly.This fact enlarges the area of potential research into the ability of fungi to survive in adverse soil conditions. The ingredients of the nutrient medium are as follows:1) pine needle extract,2)acidic malt agar.