松针褐斑病菌毒素LA-Ⅰ和LA-Ⅱ致毒活性研究

doi:10.11707/j.1001-7488.20020414

林业科学 ›› 2002, Vol. 38 ›› Issue (4): 84-88.doi: 10.11707/j.1001-7488.20020414

松针褐斑病菌毒素LA-Ⅰ和LA-Ⅱ致毒活性研究

杨斌¹ 叶建仁² 包宏⁵ 刘吉开³ 董泽军⁴

南京林业大学,南京210037；中国科学院昆明植物研究所,昆明650204

收稿日期:2001-05-08 修回日期:1900-01-01 出版日期:2002-07-25 发布日期:2002-07-25

STUDIES ON THE PHYTOTOXIC ACTIVITIES OF LA-Ⅰ AND LA-Ⅱ PRODUCING BY BROWN SPOT NEEDLE BLIGHT FUNGUS (LECANOSTICTA ACICOLA)

Yang Bin,Ye Jianren,Bao Hong,Liu Jikai,Dong Zejun

Nanjing Forestry University Nanjing 210037;Kunming Institute of Botany,The Chinese Academic of Science Kunming 650204

Received:2001-05-08 Revised:1900-01-01 Online:2002-07-25 Published:2002-07-25

摘要/Abstract

摘要：

研究比较了松针褐斑病菌(Lecanosticta acicola)两个致病毒素LA-Ⅰ和LA-Ⅱ的热稳定性、寄主专化性和最低有效浓度以及它们间的相互关系。LA-Ⅰ和LA-Ⅱ均具热稳定性,经沸水水浴加热15min仍具活性。LA-Ⅰ和LA-Ⅱ不仅能伤害易感寄主植物湿地松、火炬松,对云南松、华山松、紫茎泽兰等也有伤害作用,说明LA-Ⅰ和LA-Ⅱ均为非寄主专化性毒素。LA-Ⅰ和LA-Ⅱ配成不同浓度溶液生测,显示LA-Ⅰ对湿地松幼苗的最低有效浓度为100μg·mL^-1 ,LA-Ⅱ的最低有效浓度为500μg·mL^-1,LA-Ⅰ活性较LA Ⅱ的活性高4～5倍。LA-Ⅰ和LA-Ⅱ混合液生测表明,2个活性物质对寄主植物的伤害作用没有明显的相互促进或相互抑制作用。

关键词: 松针褐斑病菌, 毒素, 致毒活性

Abstract:

Brown spot needle blight of pine was an important disease in South China, which was caused by Lecanosticta acicola. The pathogen could produce two kinds of toxin, LA-Ⅰand LA-Ⅱ. The toxin activities and basic properties of LA-Ⅰand LA-Ⅱ were studied in the way of bioassay. The results indicated that LA-Ⅰand LA-Ⅱ could not only injure the seedlings of host pine (Pinus elliottii,P.taeda) but also make the seedlings of other plants (P.yunnanensis,P.armandii and Eupatorium sp.) wilt. So both of the two toxins were non host specific toxins(NHST). LA-Ⅰand LA-Ⅱ could keep their toxic activities for 15 minutes after boiling. The minimal effective concentrations of LA-Ⅰand LA-Ⅱ were 100?μg·mL and 500 μg·mL respectively and their activities were not very strong.LA-Ⅰand LA-Ⅱ could not affect their activities each other when they were mixed.

Key words: Lecanosticta acicola, Toxins, Toxic activity

杨斌叶建仁包宏刘吉开董泽军. 松针褐斑病菌毒素LA-Ⅰ和LA-Ⅱ致毒活性研究[J]. 林业科学, 2002, 38(4): 84-88.

Yang Bin;Ye Jianren;Bao Hong;Liu Jikai;Dong Zejun. STUDIES ON THE PHYTOTOXIC ACTIVITIES OF LA-Ⅰ AND LA-Ⅱ PRODUCING BY BROWN SPOT NEEDLE BLIGHT FUNGUS (LECANOSTICTA ACICOLA)[J]. Scientia Silvae Sinicae, 2002, 38(4): 84-88.

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松针褐斑病菌毒素LA-Ⅰ和LA-Ⅱ致毒活性研究

STUDIES ON THE PHYTOTOXIC ACTIVITIES OF LA-Ⅰ AND LA-Ⅱ PRODUCING BY BROWN SPOT NEEDLE BLIGHT FUNGUS (LECANOSTICTA ACICOLA)

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