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林业科学 ›› 2011, Vol. 47 ›› Issue (10): 111-115.doi: 10.11707/j.1001-7488.20111017

• 论文 • 上一篇    下一篇

杂种落叶松不同子代针叶内抗氧化酶活性的比较

严善春1, 门丽娜1, 石蕾2, 鲁艺芳1   

  1. 1. 东北林业大学林学院林木遗传育种国家重点实验室 哈尔滨 150040;2. 内蒙古自治区环境科学研究院环境规划研究所 呼和浩特 010020
  • 收稿日期:2010-03-21 修回日期:2010-11-22 出版日期:2011-10-25 发布日期:2011-10-25

Comparative Analysis of Antioxidase Activities in Needles of Different Larch Hybrids

Yan Shanchun1, Men Lina1, Shi Lei2, Lu Yifang1   

  1. 1. State Key Lab. of Tree Genetic and Breeding, Northeast Forestry University Harbin 150040;2. Environmental Planning Institute, Inner Mongolia Environmental Science Academy Hohhot 010020
  • Received:2010-03-21 Revised:2010-11-22 Online:2011-10-25 Published:2011-10-25

摘要:

为研究不同品种杂种落叶松针叶内抗氧化酶活性的差异,用紫外分光光度法对日本落叶松、兴安落叶松和长白落叶松的杂交后代,共2代7个家系的杂种落叶松针叶内的过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性及其差异进行比较分析。结果表明:2代7个家系杂种落叶松针叶内的CAT、POD和SOD的活性差异极显著(P <0.01),F1代CAT、SOD的活性普遍高于F2代,POD活性低于F2代。在抗氧化酶方面,F1代杂种优势高于F2代,F1代日3×兴2、F2代日12×兴9等具有较好的潜在抗虫性; 结合其他抗虫生理指标,该研究结果在选育落叶松抗虫品种过程中有参考价值。

关键词: 杂种落叶松, 超氧化物歧化酶, 过氧化氢酶, 过氧化物酶

Abstract:

Antioxidases are important defensive substance in plants, and can maintain balance of oxidative metabolisms in vivo to ensure healthy growth of plants. In this study, the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in needles of hybrids between Larix kaempferi, L. gmelinii and L. olgensis were assayed with UV-spectrometry to investigate variation in the enzymes among the hybrids. The results showed that there were significant difference (P<0.01) in SOD, CAT and POD activities among 7 families hybrids of two generations. Though POD activity of F1 generation was lower than F2 generation, activities of the CAT or SOD were higher than F2 generation, suggesting that heterosis of F1 generation was greater than F2 generation. Additionally, some hybrids, such as F1 generation of L. kaempferi 3 ×L. gmelinii 2, and F2 generation of L. kaempferi 12 ×L. gmelinii 9, expressed strong resistance to pests and could be used as resistance resources against larch pests.

Key words: larch hybrids, catalase, peroxidase, superoxide dismutase

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