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林业科学 ›› 2011, Vol. 47 ›› Issue (4): 182-189.doi: 10.11707/j.1001-7488.20110429

• 研究简报 • 上一篇    下一篇

常用农药对5种昆虫细胞系的毒力测定及分析

张欣1,2, 冯颖1,2, 丁伟峰1,2, 马涛1,2, 刘荣2, 马艳2   

  1. 1. 中国林业科学研究院资源昆虫研究所 昆明 650224;2. 国家林业局资源昆虫培育与利用重点实验室 昆明 650224
  • 收稿日期:2009-10-22 修回日期:2010-08-18 出版日期:2011-04-25 发布日期:2011-04-25
  • 通讯作者: 冯颖

Measurement and Analysis of Cytotoxicity of 5 Insect Cell Lines Induced by Some Pesticides

Zhang Xin1,2, Feng Ying1,2, Ding Weifeng1,2, Ma Tao1,2, Liu Rong2, Ma Yan2   

  1. 1. Research Institute of Resource Insects,CAF Kunming 650224;2. Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration Kunming 650224
  • Received:2009-10-22 Revised:2010-08-18 Online:2011-04-25 Published:2011-04-25

关键词: 毒力, 昆虫细胞系, 农药, 二甲苯, 致死率

Abstract:

The toxicity of lambda-cyhalothrin、beta-cypermethrin plus emulsion, E,C、alathion、azadirachtin and xylene at the different concentration and treating time was studied with five insect cell lines, applying Blackman viability analysis instrument. The results showed that the four types of pesticides had toxic effect on the cell lines, while xylene at three concentrations had no obvious toxic effect on the cell lines. The tests indicated that azadirachtin was significantly more toxic than other pesticides, then in turn were beta-cypermethrin plus emulsion,E,C, alathion, and lambda-cyhalothrin. The five cell lines responded differentially to the same testing concentration of same pesticide, and had different sensitivity to the chemicals. Amonge them, cell line Sf 9 was more sensitive than other cell lines, followed in turn by NIAS-MaBr-85,SL2, NIH-SaPe-4 and C6/36. The results indicated that the sensitivity of insect cell lines to the pesticides was in accord to the mechanism of pesticides, and the insect cell lines used for the determination of pesticide cytotoxicity is feasible.

Key words: cytotoxicity, insect cell lines, pesticide, xylene, fatality rates

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