欢迎访问林业科学,今天是

林业科学 ›› 2001, Vol. 37 ›› Issue (zk): 177-180.doi: 10.11707/j.1001-7488.2001S132

• 论文及研究报告 • 上一篇    下一篇

应用磁化水深层培养绿僵菌液生分生孢子初探

宋漳 江英成   

  1. 福建农林大学林学院,南平353001;福建省上杭县森防检疫站,上杭364200
  • 收稿日期:2001-01-08 修回日期:1900-01-01 出版日期:2001-11-25 发布日期:2001-11-25

PRELIMINARY STUDY ON SUBMERGED CONIDIA OF METARRHIZIUM ANISOPLIAE AND METARRHIZIUM GUIZHOUENSE CULTURED BY MAGNETIZED WATER

Song Zhang,Jiang Yingcheng   

  1. Forestry College of Fujian Agriculture and Forestry University Nanping 353001;Forest Disease and Insect Pest Control and Quarantine Station of Shanghang County Shanghang 364200
  • Received:2001-01-08 Revised:1900-01-01 Online:2001-11-25 Published:2001-11-25

关键词: 磁化水, 绿僵菌, 液体深层培养, 液生分生孢子, 马尾松毛虫, 毒力

Abstract:

strains of Metarrhizium anisopliae and Metarrhizium guizhouense were cultivated in submerged culture with magnetized water, and the biomagnetic effects of magnetized water on submerged sporulation of Metarrhizium anisopliae and Metarrhizium guizhouense were preliminarily studied. The results indicated that the magnetized water affected the production of submerged conidia. The output of submerged conidia were increased significantly in proper magnetized water medium. In addition, the infectivity of submerged conidia of M337 strain was tested in laboratory. The test showed that there were no significant differences in the toxicity to the 3rd~4th instar larvae of Dendrolimus punctatus between Submerged conidia harvested from medium of magnetized water and those from medium of non-magnetized water. It is hoped that the results may be used as the basis for the study on large scale of submerged conidia of Metarrhizium anisopliae and Metarrhizium guizhouense cultivated by magnetized water.

Key words: Magnetized water, Metarrhizium anisopliae, Metarrhizium guizhouense, Submerged culture, Submerged conidia, Dendrolimus punctatus, Toxicity