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林业科学 ›› 2011, Vol. 47 ›› Issue (5): 23-30.doi: 10.11707/j.1001-7488.20110504

• 论文 • 上一篇    下一篇

基于SSR和SRAP标记的簸箕柳×绵毛柳遗传框架图

刘恩英1,3, 王源秀1,2, 徐立安1, 黄敏仁1   

  1. 1. 南京林业大学 林木遗传与生物技术省部共建教育部重点实验室 南京210037;2. 安徽师范大学生命科学学院 芜湖241000;3. Forest Biotechnology Group, North Carolina State University Raleigh NC 27695 USA
  • 收稿日期:2010-01-28 修回日期:2010-03-24 出版日期:2011-05-25 发布日期:2011-05-25
  • 通讯作者: 徐立安

A Genetic Frame Map of Salix suchowensis × S. erioclada Based on SSR and SRAP Markers

Liu Enying1,3, Wang Yuanxiu1,2, Xu Li1, Huang Minren1   

  1. 1. Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education Nanjing Forestry University Nanjing 210037;2. College of Life Sciences, Anhui Normal University Wuhu 241000;3. Forest Biotechnology Group, North Carolina State University Raleigh NC 27695 USA
  • Received:2010-01-28 Revised:2010-03-24 Online:2011-05-25 Published:2011-05-25

摘要:

以簸箕柳× 绵毛柳的F1代为作图群体,构建1份柳树遗传连锁框架图,该图谱包含117个标记(56个SSR,54个SRAP,5个SCAR和2个性别标记),图谱总图距为1 631.4 cM,标记间平均图距为14.1 cM。估算柳树基因组的大小为2 261.39 cM,总的图谱覆盖率为72.14%。2个性别标记Sex-F和Sex-M被定位于该图谱第1和第17连锁群上,在第1连锁群上存在与雌性性别共分离的标记位点SCAR_AE08-780-5。通过基因组图谱初步比较研究发现,柳树框架图与毛果杨全基因组之间共有46个同源标记、15个同源连锁群,80.95%的同源标记具有共线性,这说明杨柳科基因组在进化过程中具有较高的保守性。

关键词: 簸箕柳, 绵毛柳, SRAP, SSR, 遗传图谱

Abstract:

Based on a mapping population derived from Salix suchowensis × S. erioclada F1 family, a genetic frame map of willow with 117 markers (56 SSR, 54 SRAP, 5 SCAR and 2 sex markers) was constructed in this study. The map was totally 1 631.4 cM with average distance between markers of 14.1cM and covered 72.14% of the total length of the genome which was estimated to be as long as 2 261.39 cM. Two sex markers were located on group 1 and 17 and the marker SCAR_AE08-780-5 was co-segregated with the female marker Sex-F on group 1. Alignment of this frame map to the Populus trichocarpa physical map revealed 46 homologous markers, 15 homological linkage groups and 34 of 42 homologous markers (80.95%) were at high co-linearity. Overall, the genome of the Salicaceae family was well conserved in the course of evolution.

Key words: Salix suchowensis, Salix erioclada, SRAP, SSR, genetic linkage map

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