铅胁迫下马尾松微核率与生理指标变化的相关分析

doi:10.11707/j.1001-7488.20100812

林业科学 ›› 2010, Vol. 46 ›› Issue (8): 78-83.doi: 10.11707/j.1001-7488.20100812

铅胁迫下马尾松微核率与生理指标变化的相关分析

吴若菁¹，庄捷²，陈清松³，陈文萍¹

1.福建师范大学生命科学学院福州 350108;2.福建商贸学校福州 350007;3.福建师范大学化学与材料学院福州 350007

收稿日期:2009-10-19 修回日期:2010-06-02 出版日期:2010-08-25 发布日期:2010-08-25

Correlations between of Micronucleus Rate and Physiological Changes of Pinus massoniana in Response to Lead Stress

Wu Ruojing¹;Zhuang Jie²;Chen Qingsong³;Chen Wenping¹

1.College of Life Sciences，Fujian Normal University Fuzhou 350108; 2.Fujian Business School Fuzhou 350007;3.College of Chemistry and Materials Sciences，Fujian Normal University Fuzhou 350007

Received:2009-10-19 Revised:2010-06-02 Online:2010-08-25 Published:2010-08-25

摘要/Abstract

摘要：

利用微核技术、TBA显色法、电导率仪法、NBT还原法和愈创木酚法等研究技术，对铅胁迫下马尾松染色体损伤及生理指标变异进行相关分析的研究。结果表明： 1）不同浓度的铅胁迫对马尾松微核率的影响经t检验与对照相比均达到极显著差异。2）随铅浓度的加大，微核率、MDA、细胞伤害度、SOD和POD值逐渐上升，在铅浓度为2 000 mg·L^-1时，MDA、细胞伤害度、SOD和POD值达到最高值，超过这个阈值，随着铅浓度的逐渐加大，MDA、细胞伤害度、SOD和POD值开始下降，说明马尾松的自身的抗氧化酶系统对Pb²⁺毒害的保护作用有限度。3）铅浓度与微核率回归采用Cubic模式；微核率与伤害度，微核率与MDA，MDA与伤害度，MDA与POD回归采用S模式； MDA与SOD，SOD与POD回归采用Inverse模式回归时，预测相互间变化效果最佳，相关性可以达到显著或极显著水平。

关键词: 马尾松, 幼苗, 铅胁迫, 微核率, 生理指标, 回归分析

Abstract:

This paper employed several research techniques, including micronucleus technology, TBA reaction method, conductivity meter method, NBT reduction and guaiacol method, to investigate changes in physiological parameters related to chromosome damage of Pinus massoniana under lead stress and analyzed the correlations between the traits. The results showed that: 1) Different concentration leads significantly affected micronucleus rate of P. massoniana compared with the untreated control by t test. 2) With increasing concentration of lead, micronucleus rate, MDA, cell injury, SOD and POD activities gradually increased. The treatment with 2 000 mg·L^-1 lead, brought about the highest MDA content, cell injury, SOD and POD activities, and over the concentration of lead, MDA content, cell injury, SOD and POD activities began to decline with gradually increasing lead concentration, suggesting that the antioxidant enzyme systems had limited capacity in protecting the plants from Pb²⁺ toxicity. 3) Regression analyses showed that correlation between lead concentrations and the micronucleus rate fitted a Cubic model; correlations between micronucleus rate and degree of damage, between micronucleus rate and MDA, between MDA and the degree of damage, between MDA and POD took a S model; and MDA and SOD, SOD and POD regressions with an Inverse model best predicted changes in each other.The relationships were significantly or very significantly correlated.

Key words: Pinus massoniana, seedlings, lead stress, micronucleus rate, physiological index, regression analysis

吴若菁;庄捷;陈清松;陈文萍. 铅胁迫下马尾松微核率与生理指标变化的相关分析[J]. 林业科学, 2010, 46(8): 78-83.

Wu Ruojing;Zhuang Jie;Chen Qingsong;Chen Wenping. Correlations between of Micronucleus Rate and Physiological Changes of Pinus massoniana in Response to Lead Stress[J]. Scientia Silvae Sinicae, 2010, 46(8): 78-83.

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铅胁迫下马尾松微核率与生理指标变化的相关分析

Correlations between of Micronucleus Rate and Physiological Changes of Pinus massoniana in Response to Lead Stress

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