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林业科学 ›› 2004, Vol. 40 ›› Issue (2): 55-60.doi: 10.11707/j.1001-7488.20040210

• 论文及研究报告 • 上一篇    下一篇

美洲黑杨×青杨木材性状QTLs定位研究

黄秦军 苏晓华 黄烈健 张志毅   

  1. 中国林业科学研究院林业研究所,北京100091;中国林业科学研究院热带林业研究所,广州510520;北京林业大学,北京100083
  • 收稿日期:2003-11-20 修回日期:1900-01-01 出版日期:2004-03-25 发布日期:2004-03-25

QTLs Mapping for Wood Properties of Populus deltoides×P. cathayana

Huang Qinjun,Su Xiaohua,Huang Liejian,Zhang Zhiyi   

  1. Research Institute of Forestry, CAF Beijing100091;Research Institute of Tropical Forestry, CAF Guangzhou510520;Beijing Forestry University Beijing100083
  • Received:2003-11-20 Revised:1900-01-01 Online:2004-03-25 Published:2004-03-25

摘要:

利用来自87个F2代单株的810个标记(784个AFLP标记和26个SSR标记)构建了美洲黑杨×青杨杂种分子连锁遗传图谱。该图谱包括19个主要连锁群,共有368个标记(356个AFLP标记和12个SSR标记)。框架图总图距为3382.4cM ,平均标记间距为9.69cM。利用区间作图法在LOD≥2 ,检测区间为2cM条件下检测到8个与木材性状有关的QTLs。其中与木材基本密度关联的3个QTLs贡献率分别为27.1 %、27.1 %、25.5 % ,与微纤丝角关联的QTLs贡献率为18.6 % ,与纤维长度关联的QTLs贡献率为48.9% ,与纤维宽关联的3个QTLs贡献率分别为17.4 %、27.4 %和48.3%。这8个QTLs遗传作用方式均为超显性,其中只有2个与基本密度关联的QTLs来自于美洲黑杨,其余6个均来自于青杨。

关键词: 美洲黑杨×, 青杨, 木材性状, QTLs定位

Abstract:

Based on 810 molecular markers(784 AFLP markers and 26 SSR markers),obtained from the 87 individuals of F2 population from Populus deltoides×P. cathayana , a hybrid molecular linkage map was constructed. In this map, 356 AFLP makers and 12 SSR markers, 368 makers in total, were distributed in the 19 major groups. This map covered 3 382.4 centiMorgan (cM) of the genome in length, and the average distance between two markers was 9.69cM. With the LOD≥2.0 and scan scale=2.0cM, total 8 QTLs for wood properties were obtained by using interval mapping (IM) in this map. The phenotypic variation contributions (PVCs) of 3 QTLs for wood basic density were 27.1%,27.1% and 25.5%,respectively; PVC of 1 QTL for microfibre angle was 18.6%;PVC of 1 QTL for fibre length was 48.9%; and PVCs of 3 QTLs for fibre width were 17.4%,27.4% and 48.3%. Gene actions of all QTLs were overdominance. The two original parents made different contributions to the QTLs of their hybrid progeny. The P. deltoides parent contributed 2 QTLs, which associated with wood basic density, and P. cathayana contributed 6 QTLs to their hybrid progeny.

Key words: Populus deltoides×, P. cathayana, Wood property, QTLs mapping