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林业科学 ›› 1999, Vol. 35 ›› Issue (1): 47-52.

• 论文及研究报告 • 上一篇    下一篇

华东地区豆科树种根瘤菌多样性的研究

陈景荣1,韩素芬2   

  1. 1. 福建省森林病虫害防治检疫总站 福州 350003
    2. 南京林业大学 南京 210037
  • 收稿日期:1998-06-08 出版日期:1999-01-25 发布日期:1999-01-25
  • 基金资助:
    国家自然科学基金(39670601)

DIVERSITY OF NODULE BACTERIA ISOLATED FROM THE ROOT NODULES OF LEGUMINOUS TREES IN EAST CHINA

Jingrong Chen1,Sufen Han2   

  1. 1. Forest Pest Quarantine & Control Station of Fujian Province Fuzhou 350003
    2. Nanjing Forestry University Nanjing 210037
  • Received:1998-06-08 Online:1999-01-25 Published:1999-01-25

摘要:

华东地区51个豆科树种(含羞草亚科5属,蝶形花亚科18属)根瘤中分离获得的66个菌株经形态、生理生化、抗逆性等性状测定,对42个性状进行了聚类分析,除92-78-2菌株外, 65个菌株在0.75的相似水平上可划分为6个菌群。根据菌株的生长速度, 6个菌群可以分为快生和慢生两个类群,但两类群的某些性状与目前根瘤菌分类系统中的分属辨别特征不完全符合,如部分菌株能利用其淀粉。聚类分析结果还表明,菌群划分的主要依据是表型特征,与寄主的种类、来源没有相关性。66个菌株在形态、生理生化、抗逆性以及交叉结瘤试验结果存在明显差异,表明了豆科树种根瘤菌的多样性。

关键词: 华东地区, 豆科树种根瘤菌, 多样性

Abstract:

Sixty-six rhizobial strains, isolated from root nodules of fifty-one species of woody legumes (five genera in subfamily Mimosoideae and eighteen genera in subfamily Papilionoideae) in East China, were studied by performing a fuzzy analysis of 42 phenotypic characteristics. Six clusters were formed below the boundary level of 0.75 average distance except 92-78-2 strain. We consider that six clusters should belong to the fast growers and slow growers. However, the properties of the two growers are correspond incompletely with the differential characters of the 4 genera in the rhizobial taxonomic system, for example, some strains can utilize starch as the sole carbon source. The results of numerical analysis also suggest that the primary basis of the division of the clusters is the phenotypic characteristics, and has nothing to do with the category and origin of the hosts. Our results indicate that tree rhizobia are very diverse with respect to their physiological and biochemical properties.

Key words: East China, Rhizobia from woody legumes, Diversity