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›› 1994, Vol. 30 ›› Issue (4): 320-324.

• 论文及研究报告 • 上一篇    下一篇

表达苏云金杆菌δ-内毒素蛋白基因大肠杆菌对四种林业害虫作用的生物测定

王学聘 戴莲韵 陈倩 田颖川   

  1. 中国林业科学研究院林业研究所,北京100091;中国科学院微生物研究所,北京100080
  • 收稿日期:1993-11-01 修回日期:1900-01-01 出版日期:1994-07-25 发布日期:1994-07-25

BIOASSAY OF ESCHERICHIA COLI OF EXPRESSING BACILLUS THURINGIENSIS δ-ENDOTOXIN GENE AGAINST FOREST PESTS

Wang Xuepin,Dai Lianyun,Chen Qian,Tian Yingchuan   

  1. The Research Institute of Forestry, CAF Beijing 100091;Institute of Microbiology, Academia Sinica. Beijing 100080
  • Received:1993-11-01 Revised:1900-01-01 Online:1994-07-25 Published:1994-07-25

摘要: 四株表达苏云金杆菌δ-内毒素蛋白基因的大肠杆菌对林业主要害虫杨扇舟蛾Clostera anachoreta(Fabricius),马尾松毛虫Dendrolimus punctatus(Walker),舞毒蛾Lymantria dispar(Linnaeus),杨尺蠖Apocheima cinerarius(Erschoff)幼虫作用进行了室内生物测定,结果表明:该四株菌对上述四种昆虫幼虫均表现出较高的杀虫活性,而且能显著抑制昆虫幼虫的生长发育。

关键词: 苏云金杆菌, δ-内毒素, 生物测定

Abstract: Bioassay of four Escherichia coli of experessing Bacillus thuringiensis δ-endotoxin gene against forest pests (Lymantria dispar, Apocheimacinerarius, Dendrolimus punctatus, Clostera anachoreta) showed: The four cloned strains had high toxicity against above-mentioned pests and could inhibit growth and development of the pests.

Key words: Bacillus thuringiensis, δ-endotoxin, Bioassay