关键词: mRNA运输, Bt毒蛋白运输, 嫁接, 抗虫性

Abstract:

In this paper poplar741 and Pb29 transgenic poplar741with Btcry1Ac gene were grafted as scion and stock mutually, to investigate whether mRNA and protein of Bt gene would be transported between the stock and scion by means of RT-PCR and ELISA techniques. The result of RT-PCR test showed that mRNA of Bt gene was not detected in the branch and leaf of poplar741 no matter its material was used as scion or stock, which suggested that mRNA of Bt gene was not transported between the stock and scion. The result of ELISA test showed that Bt toxin protein was detected in the leaf, phloem, xylem and pith of poplar741 in the grafted plants, which demonstrated that Bt toxin protein was transported between the stock and scion in the grafted plants. To find out any influence of grafting on larvae of Clostera anachoreta, the larvae were fed indoors on the leaves of grafted scion. The results showed that grafting the poplar741 to transgenic ones could increase the death rate, extend the larval developmental period of C. anachoreta, and express certain resistance to insects.

Key words: transportation of mRNA, transportation of Bt toxin protein, grafting, insect-resistance