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林业科学 ›› 2011, Vol. 47 ›› Issue (1): 124-134.doi: 10.11707/j.1001-7488. 20110120

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植物非生物逆境胁迫DREB/CBF转录因子的研究进展

李科友1, 朱海兰2   

  1. 1. 西北农林科技大学 生命科学学院 杨凌 712100;2. 西北农林科技大学林学院 杨凌 712100
  • 收稿日期:2010-08-02 修回日期:2010-10-06 出版日期:2011-01-25 发布日期:2011-01-25

Research Progress of DREB/CBF Transcription Factor in Response to Abiotic-Stresses in Plants

Li Keyou1, Zhu Hailan2   

  1. 1. College of Life Sciences,Northwest A & F University Yangling 712100;2. College of Forestry, Northwest A & F University Yangling 712100
  • Received:2010-08-02 Revised:2010-10-06 Online:2011-01-25 Published:2011-01-25

摘要:

综述近十几年来,特别是近5年来国内外DREB/CBF转录因子的研究进展, 主要包括DREB/CBF转录因子的结构特点, DREB/CBF 基因的克隆、表达调控及其在植物抗逆基因工程中的作用,以及DREB/CBF转录因子研究中的问题与展望,旨在为植物抗逆育种提供理论依据。DREB/CBF类转录因子即干旱应答元件结合蛋白质/C-重复序列结合子,是AP2/EREBP转录因子家族的一个亚家族, 拥有保守的AP2结构域, 能够特异性地与抗逆基因启动子区域的DRE/CRT顺式作用元件相结合,在干旱、低温和高盐等条件下调节一系列下游逆境应答基因的表达, 是植物逆境适应中的关键性调节因子。目前,已从拟南芥、欧洲油菜、水稻、玉米、小麦、陆地棉、大豆和番茄等几十种植物中分离并鉴定出调控干旱、高盐及低温耐性的 DREB/CBF 基因, 并利用这些基因得到抗逆性增强的拟南芥、欧洲油菜、番茄、小麦以及杨树等转基因植株。转基因结果表明,DREB/CBF转录因子家族在植物抗逆品种改良中具有重要的应用价值。

关键词: DREB/CBF, 转录因子, DRE/CRT顺式作用元件, 转基因植物, 抗逆育种

Abstract:

The research progress on DREB/CBF (Dehydration Responsive Element Binding Protein/C-repeat Binding Factor) transcription factors in last decades especially in recent five years is reviewed,mainly including the structural features of DREB/CBF transcription factors,the cloned DREB/CBF genes and their expression regulations,the application of DREB/CBF genes in gene engenieering for improving plant stress resistance,as well as the existing problems and the future prospective of DREB/CBF to provide reference for plant stress-resistant breeding. DREB/CBF transcription factor, with a typical AP2/EREBP DNA-binding domain, could specifically bind to the DRE/CRT (Dehydration Responsive Element /C-repeat) cis-acting element and activate a lot of the expression of stress inducible genes under dehydration, low temperature and saline conditions, and hence increase plants’ tolerance to environmental stresses. According to the published literature in last decades,a vast number of DREB/CBF transcription factor genes have been isolated and characterized from a variety of plants such as Arabidopsis thaliana , Brassica napus , Oryza sativa , Zea mays , Triticum aestivum , Gossypium hirsutum , Glycine max and Lycopersicon esculentum . Over-expression of these DREB/CBF cDNAs in Arabidopsis thaliana , Brassica napus , Lycopersicon esculentum , Triticum aestivum and Populus could greatly enhance stress tolerance of these transgenic plants, which indicated the importance of DREB/CBF transcription factors in plant stress-resistant breeding.

Key words: DREB/CBF, transcription factor, DRE/CRT cis-acting element, transgenic plant, stress-resistant breeding

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